GATA11 Antibody

Overview of GATA1 Antibodies

GATA1 antibodies are immunological tools designed to detect and study the GATA-binding factor 1, a zinc-finger transcription factor essential for erythroid, megakaryocytic, eosinophil, and mast cell development . These antibodies enable researchers to investigate GATA1's role in gene regulation, differentiation, and disease pathogenesis.

Key Applications of GATA1 Antibodies

GATA1 antibodies are validated for multiple experimental techniques:

Specificity Testing

• Knockout Validation: Antibodies like ab181544 show no signal in GATA1 knockout K562 cells, confirming specificity .

• Cross-Reactivity: Clone D52H6 (CST #3535) reacts with human, mouse, and rat GATA1 .

Functional Insights from Research

• Basophil Regulation: GATA1 knockdown reduces IL-4 production in basophils, impairing anti-parasitic immunity .

• Megakaryopoiesis: Genome-wide studies identify GATA1 co-binding with RUNX1 and FLI1 at enhancers regulating megakaryocyte genes .

• Disease Links: Mutations in GATA1 are associated with X-linked thrombocytopenia and leukemia .

Research Findings Using GATA1 Antibodies

• Transcriptional Networks: GATA1 collaborates with SCL and RUNX1 at enhancers to regulate megakaryocyte-specific genes .

• Therapeutic Potential: IgA antibodies (a class regulated by GATA1) show anti-tumor activity in preclinical models .

• Pathogenic Mechanisms: Impaired GATA1 binding at β-globin HS2 reduces ε-globin transcription by 93%, highlighting its role in globin switching .

Limitations and Considerations

• Species Specificity: Some antibodies (e.g., ab109115) are human-specific .

• Batch Variability: Commercial lots may differ; validation in knockout models is critical .