GATA14 Antibody

Definition and Biological Context

GATA-4 Antibody is an immunological tool designed to detect GATA4, a zinc-finger transcription factor critical for cardiac development, hepatocyte differentiation, and reproductive organogenesis . This antibody binds specifically to GATA4's conserved DNA-binding domain, enabling researchers to study its expression, localization, and functional interactions .

Cardiac Regeneration

• GATA4-targeted compounds (e.g., 3i-1000 derivatives) modulate atrial/ventricular subtype differentiation in stem cells:

  • Increased ventricular GFP+ cells by 15-20% (P < 0.05) in dual reporter assays .

  • Synergistic effects observed with BET inhibitors, enhancing chamber-specific gene expression .

Disease Associations

• Liver Fibrosis: GATA4 overexpression in hepatic stellate cells induces fibrosis regression via deactivation .

• Cancer: Aberrant expression noted in hepatocellular carcinoma (HepG2) but absent in breast cancer (MCF-7) .

Validation Data

• Western Blot: Strong bands in HepG2 cells, mouse heart, and rat liver .

• Immunohistochemistry: Nuclear/cytoplasmic staining in human liver tissue .

Limitations and Controversies

• Species Specificity: Most clones lack reactivity in non-mammalian models .

• Functional Redundancy: GATA4 knockdown in adult cardiomyocytes showed compensatory mechanisms, challenging earlier neonatal cell findings .