GATA18 Antibody
Description
Introduction to GATA18 and Its Role in Biological Systems
GATA18 is a transcription factor involved in cellular differentiation and development, particularly in immune and hematopoietic systems. While the term "GATA18 Antibody" is not explicitly defined in the provided literature, antibodies targeting GATA18 or its interacting partners (e.g., SAP05) are critical tools for studying its function. These antibodies enable detection, localization, and functional analysis of GATA18 in research and therapeutic contexts.
Degradation Mechanism
SAP05 recruits proteasomes via AtRPN10, a component of the 19S regulatory particle. Mutations in AtRPN10 (E31A, L69T, L73D) abolish SAP05 binding, halting GATA18 degradation .
Isothermal Titration Calorimetry (ITC)
ITC assays revealed:
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SAP05-GATA18 interaction: Exothermic reaction with Kd = 2.7 μM .
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SAP05-SPL5 interaction: Similar affinity (Kd = 2.6 μM), indicating shared binding motifs .
GST Pull-Down Assays
| AtRPN10 Mutant | SAP05 Binding | Impact on GATA18 Degradation |
|---|---|---|
| E31A | No | Abolished |
| L69T | No | Abolished |
| L73D | No | Abolished |
Clinical and Therapeutic Implications
While no direct therapeutic applications of GATA18 antibodies are reported, insights into SAP05-GATA18 interactions suggest potential targets for modulating transcriptional regulation. For example:
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Cancer therapy: Disrupting SAP05-GATA18 degradation could alter transcriptional programs in tumors .
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Immune modulation: GATA18’s role in immune cell development may be exploitable for immunotherapies .
Methodological Applications of Antibodies in GATA18 Research
Antibodies are integral to studying GATA18:
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Western blot: Detects GATA18 protein levels post-SAP05 interaction .
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Structural studies: Facilitates crystallization of SAP05-GATA18 complexes for X-ray crystallography .
Future Directions and Research Gaps
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Specific GATA18 antibodies: Development of high-affinity monoclonal antibodies for precise detection.
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In vivo models: Testing SAP05-GATA18 interactions in disease-relevant contexts.
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Therapeutic targeting: Exploring small molecules or biologics to modulate GATA18 stability .
References SAP05-mediated ubiquitin-independent degradation of GATA18 and SPL family proteins in Arabidopsis. GAT1 antibody (20298-1-AP) – Proteintech (unrelated to GATA18 but included for antibody methodology context).
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
GATA18 plays a crucial role in the development of both flower and shoot apical meristem (SAM), particularly in establishing organ boundaries within these structures. Its function in this process likely involves controlling the number and position of WUS-expressing cells.
In conjunction with AGO10/PNH, GATA18 co-regulates the organization of the shoot apical meristem. It further contributes to floral organ development by promoting the expression of JAG and NPR5/BOP2.
GATA18 modulates cytokinin homeostasis at organ boundaries through the regulation of CKX3 expression. Its involvement extends to cell proliferation and differentiation. Additionally, it is essential for positioning the inductive proembryo boundary by regulating gene expression and for early embryonic development.
In collaboration with GIF1/AN3, GATA18 mediates cotyledon identity by preventing ectopic root formation through the repression of PLT1 expression.
- HAN functions as a key repressor in regulating floral development. It does so through regulatory networks involving genes in the GATA3 family, alongside genes associated with hormone action and floral organ specification. PMID: 23335616
- Research indicates that establishing cotyledon identity requires both GIF1 and HANABA TARANU to repress ectopic root formation by suppressing PLETHORA1 expression. PMID: 22669825
- Mutations in HAN lead to a coordinated apical shift in gene expression patterns. This reveals that HAN regulates transcription in the basal proembryo. [GATA factor HANABA TARANU] PMID: 20643354
Q&A
Here’s a structured, research-focused FAQ for GATA18 antibody applications in academic settings, synthesized from peer-reviewed methodologies and validation frameworks:
Advanced Research Questions
How do I resolve contradictory results between GATA18 antibodies in mechanistic studies?
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Functional redundancy tests: Combine siRNA knockdown with antibody-based detection to assess target specificity .
What multiplex assay designs minimize cross-reactivity with GATA18 antibody?
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Use isotype-selective secondary antibodies:
Primary Host Secondary Conjugate Compatible Targets Rabbit IgG AF488 (anti-rabbit) GATA18 Mouse IgG2a AF594 (anti-mouse) Housekeeping protein -
Validate signal separation via spectral unmixing and single-antibody controls .
How do batch-to-batch variations impact longitudinal studies using GATA18 antibody?
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Standardize workflows:
Methodological Best Practices
Table 1: Critical validation parameters for GATA18 antibody applications
| Application | Key Controls | Acceptable Criteria |
|---|---|---|
| Western Blot | KO lysate, recombinant protein | Band at expected MW; no KO signal |
| IF/IHC | Isotype control, KO tissue | Subcellular localization matches |
| Flow Cytometry | Fc block, compensation controls | Clear population separation |
