GATA20 Antibody

Overview of GATA-2 Antibodies

GATA-2 is a zinc-finger transcription factor critical for hematopoiesis, endothelial cell development, and stem cell maintenance. GATA-2 antibodies are essential tools for detecting its expression and activity in research and diagnostics. These antibodies are typically raised against recombinant human or mouse GATA-2 protein fragments (e.g., Ala15-Thr279 in human isoforms) and validated for Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP).

Table 2: Antibody Performance in Western Blotting

Critical Notes:

• Band Variability: Detection bands range from 51–58 kDa, likely due to post-translational modifications or isoform differences .

• Negative Controls: #79802 shows no signal in U-2 OS and Jurkat cells, confirming specificity .

Western Blotting

GATA-2 antibodies are widely used to study protein expression in hematopoietic cells (e.g., KG-1, K562) and endothelial cells (e.g., HUVECs). For example:

• AF2046 detects nuclear GATA-2 in HUVECs and SH-SY5Y neuroblastoma cells via fluorescence ICC .

• #79802 enables ChIP and CUT&RUN assays to map GATA-2 binding sites in chromatin .

Immunohistochemistry (IHC)

• AF2046 stains endothelial cell nuclei in human duodenum sections after heat-induced epitope retrieval .

• ab153820 localizes GATA-2 to the cytoplasm/nucleus in HeLa cells via IF .

Chromatin Immunoprecipitation (ChIP)

• #79802 is validated for ChIP using SimpleChIP® Enzymatic Kits, enabling studies of GATA-2 transcriptional targets .

Limitations and Considerations

• Cross-Reactivity Risks: Polyclonal antibodies may bind non-specific epitopes, necessitating blocking controls.

• Batch Variability: Users should validate antibodies in their specific experimental system.

• Storage: Most antibodies require storage at -20°C to -70°C to maintain activity .

Research Findings and Citations

• Hematopoietic Studies: MAB2046 was used in a ChIP study to investigate HbF expression linked to the HBS1L-MYB intergenic region .

• Cancer Research: GATA-2 overexpression is implicated in leukemia, with antibodies like AF2046 aiding in studying its role in KG-1 cells .