GATA23 Antibody

Biological Role of GATA23 in Plants

GATA23 is a transcription factor encoded by the AtGATA23 gene in Arabidopsis thaliana. It plays a central role in lateral root founder cell specification by regulating asymmetric cell division in xylem pole pericycle cells . Key findings include:

• Auxin Dependency: GATA23 expression is controlled by an Aux/IAA28-dependent auxin signaling pathway in the root basal meristem .

• Cellular Specificity: It marks founder cells before the first asymmetric division, aligning with oscillating auxin maxima in the root meristem .

• Functional Interactions: GATA23 interacts with PRH1 (a plant homeodomain protein) to modulate lateral root development, as shown through co-immunoprecipitation and BiFC assays .

Absence of GATA23 Antibodies in Current Literature

Despite its importance in plant biology, no commercial or experimental antibodies targeting GATA23 are documented in peer-reviewed studies or product databases. This contrasts sharply with GATA3 antibodies, which are widely used in human diagnostics (e.g., breast and urothelial cancer biomarkers) .

GATA3 Antibodies: A Potential Source of Confusion

GATA3 antibodies are extensively used in human pathology but should not be conflated with GATA23. Below are key characteristics of GATA3 antibodies for context:

4.1. Applications and Reactivity

Notes:

• GATA3 antibodies target nuclear proteins in human cells (e.g., MCF-7 breast cancer cells) .

• Reactivity with plant GATA23 has not been validated .

Research Implications and Future Directions

While GATA23 antibodies remain unavailable, researchers rely on GFP fusion constructs (e.g., GATA23-GFP in BiFC assays) to study its localization and interactions . Future efforts could focus on:

• Antibody Development: Generating plant-specific antibodies for GATA23 to enable immunohistochemical or ChIP-seq studies.

• Cross-Reactivity Testing: Investigating whether GATA3 antibodies inadvertently bind plant GATA23, though sequence divergence makes this unlikely .