GATA24 Antibody

GATA4 Antibody: Overview and Applications

GATA4 antibodies are tools used to detect and study the GATA binding protein 4 (GATA4), a transcription factor critical in cardiovascular development, liver function, and cancer biology. These antibodies are validated for applications such as Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FC), and Immunoprecipitation (IP).

Key Applications and Dilutions

Antibody Specificity and Reactivity

GATA4 antibodies exhibit species-specific reactivity, primarily targeting human and mouse samples. Below are key details:

Species Reactivity

Observed Molecular Weight
GATA4 antibodies detect bands at 44–56 kDa, with variations attributed to post-translational modifications or isoforms:

Role in Cancer Biology

GATA4 antibodies have elucidated its role in hepatocellular carcinoma (HCC):

• GATA4 downregulation correlates with immunosuppressive tumor microenvironments, including increased regulatory T cells and myeloid-derived suppressor cells .

• CCL20 upregulation is linked to GATA4 loss, promoting immunosuppression and poorer survival outcomes in HCC patients .

Cellular Localization and Function

• Immunocytochemistry (ICC): GATA4 localizes to cytoplasm and nuclei in HepG2 cells (positive) and MCF-7 cells (negative) .

• Immunoprecipitation (IP): Validates GATA4 interaction networks in Huh7 (hepatocellular carcinoma) and HepG2 cells .

Critical Considerations for Experimental Use

1. Dilution Optimization: Titrate antibodies per application (e.g., WB: 1:1000–1:8000) .

2. Species Validation: Confirm reactivity across species (e.g., human vs. mouse) .

3. Storage: Most antibodies require -20°C storage for stability .

Future Directions

Emerging studies highlight GATA4’s therapeutic potential in fibrosis regression and immunotherapy. For example, GATA4 overexpression deactivates hepatic stellate cells, reducing liver fibrosis . Antibodies remain indispensable for probing these mechanisms in preclinical models.