GATA26 Antibody

Overview of GATA Family Antibodies

The GATA family (WGATAR DNA-binding motif recognition) includes six members divided into two subfamilies:

• GATA-1/2/3: Hematopoietic/endothelial regulation

• GATA-4/5/6: Cardiovascular/gastrointestinal development

Antibodies targeting these proteins enable critical research into developmental biology and disease mechanisms .

Key Applications

Research Findings:

• Mitotic Bookmarking: GATA-2 remains chromatin-bound during mitosis, essential for hematopoietic lineage commitment .

• Knockout Models: CRISPR/Cas9-mediated GATA-2 knockout in HLECs reduced miR-126 and disrupted lymphatic endothelial function .

• Reprogramming: Degradation of GATA-2 at mitotic exit delayed CD9 expression during hemogenic reprogramming (scRNA-seq data) .

Key Applications

Research Findings:

• Reprogramming Inhibition: AZD-4547 (FGFR inhibitor) reduced GATA-6 expression in embryoid bodies, correlating with Nanog upregulation (Western blot; *p < 0.001) .

• Epithelial Homeostasis: GATA-6 regulates IL6/IL10 balance in sebocytes and maintains TGFB1 signaling in skin .

• Cancer Models: Nuclear-localized GATA-6 detected in HT-29 colon adenocarcinoma cells via immunofluorescence (15 µg/mL antibody concentration) .

Comparative Analysis of Antibody Performance

Technical Considerations

• Phospho-Specificity: Anti-phospho-GATA6(Y271) (Abcepta AP3766a) requires validation in kinase signaling studies .

• Cross-Reactivity: GATA-2 antibody AF2046 detects both human and mouse isoforms, while MAB2046 shows 58 kDa bands in K562/DA3 cells .

• Mitotic Staining: Bookmarked GATA-2 targets (ZBTB16, CD9) require IF-optimized protocols .