GATC Antibody

Target Protein Overview

GATC (UniProt ID: O43716) is a 15 kDa mitochondrial protein encoded by the GATC gene (NCBI Gene ID: 283459) . It functions in:

• Transamidation of mischarged Glu-tRNA(Gln) to Gln-tRNA(Gln)

• Mitochondrial aminoacylation for protein synthesis

• Antibiotic resistance mechanisms via ribosomal mistranslation

Disease associations: Mutations in GATC are linked to Combined Oxidative Phosphorylation Deficiency 42 (COXPD42) .

Immunohistochemistry Performance

• Detects GATC in human liver (normal and cancerous) at 1:100 dilution

• Requires antigen retrieval with TE buffer (pH 9.0) or citrate buffer (pH 6.0)

Western Blot Analysis

• Clear 15 kDa band in LNCaP, A549, and HEK-293 cell lines

• Validated using KO cell lines through YCharOS protocols

Functional Studies

• GatC-GatA fusion proteins (8 kDa larger than wild-type) identified in antibiotic-resistant M. smegmatis

• Mutant GATC strains show 2-5× increased rifampicin tolerance

Diagnostic Potential

Therapeutic Antibody Development

Recent advancements in antibody characterization protocols have improved GATC antibody reliability :

• 96 antibody characterization reports published via YCharOS

• Standardized WB/IP protocols using KO cell lines

Critical Validation Considerations

1. Epitope Specificity:

   • AA 1-136 region targeted by multiple clones

   • Confirmed through recombinant protein testing

2. Cross-Reactivity:

   • Mouse-specific variants require separate validation

   • No observed cross-reactivity with bacterial GatC homologs

Emerging Research Directions

1. Role in mitochondrial disorders (COXPD42 pathogenesis)

2. Antibiotic tolerance mechanisms in mycobacteria

3. Quality control initiatives for antibody reproducibility