GATL10 Antibody
Description
GATC Antibody (Proteintech 68405-5-PBS)
Product Characteristics
| Parameter | Value |
|---|---|
| Host/Isotype | Mouse, IgG2a |
| Immunogen | GATC fusion protein (Ag19857) |
| Molecular Weight | 15 kDa (136 amino acids) |
| Reactivity | Human |
| Applications | Cytometric bead array, ELISA, multiplex assays, mass cytometry |
| Conjugate | Unconjugated (BSA/azide-free) |
| Storage | PBS buffer, -80°C |
| Validation | Matched antibody pair (MP50658-2 capture + 68405-5-PBS detection) |
This antibody is part of a conjugation-ready pair designed for high-throughput applications like multiplex immunoassays. Its specificity for human GATC protein is validated in ELISA and cytometric bead array systems .
Anti-GAT Antibodies in Immune Response Studies
Anti-GAT antibodies are historically significant in immunology for their role in studying idiotypic networks and immune regulation. Key findings include:
Idiotypic Specificity
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CGAT Idiotype: A shared idiotype observed in anti-GAT antibodies across multiple mouse strains. Induction requires "GT-related" determinants (e.g., poly-L-Glu-Tyr copolymers) .
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Fine Specificity: Antibodies from responder/nonresponder strains exhibit similar binding patterns to GAT10, despite differences in immune response (Ir) genes .
Table 1: Induction of CGAT Idiotype by Synthetic Polymers
| Polymer | Structure | CGAT Idiotype Induction | Ir Gene Control |
|---|---|---|---|
| GAT10 | L-Glu60L-Ala30L-Tyr10 | Yes | H-2-linked |
| GT | L-Glu51L-Ala34L-Tyr15 | Yes | Distinct Ir |
| GA | L-Glu60L-Ala40 | No | – |
Challenges in Antibody Characterization
The "antibody characterization crisis" highlights issues with specificity and reproducibility, particularly for commercial reagents . Key insights:
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Performance Variability: ~50–75% of commercial antibodies targeting specific proteins fail validation in Western blot or immunofluorescence .
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KO Cell Line Controls: Critical for validating antibody specificity; recombinant antibodies often outperform monoclonal/polyclonal counterparts .
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Industry Partnerships: Collaborative efforts (e.g., YCharOS, OGA initiatives) aim to standardize validation and remove underperforming reagents from catalogs .
Research and Diagnostics
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ELISA/IHC/Western Blot: Used to detect GAT proteins in human samples .
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Immunoprecipitation: Purification of GATC homologs for structural studies .
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Therapeutic Targets: While not directly therapeutic, anti-GAT antibodies inform strategies for targeting bacterial homologs in infections .
Critical Considerations
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Validation Requirements: Always validate antibodies in-house for specific applications (e.g., ELISA vs. IHC) .
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Germline Bias: Antibody diversity is constrained by germline gene segments, necessitating careful selection for target specificity .
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Data Transparency: Prioritize vendors that openly share validation data (e.g., KO cell line results) .
