POP2 Antibody
Description
Applications in Research
The POP2 antibody has been utilized to investigate POPDC2’s role in:
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Cardiac Function: Localizing POPDC2 in human heart tissue to study its involvement in maintaining cardiac rhythm .
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Ion Channel Regulation: Exploring POPDC2’s interaction with potassium channels (e.g., KCNK2) and its modulation via cAMP-binding .
Key Findings:
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Immunohistochemical Validation: Demonstrated strong staining in human heart muscle tissue, confirming POPDC2’s cardiac localization .
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Functional Insights: Suggested POPDC2 enhances cell surface expression of KCNK2, thereby influencing electrical activity in cardiomyocytes .
Mechanism of Action
While the antibody itself does not modulate biological activity, its target (POPDC2) functions as a regulatory protein:
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cAMP-Binding: Mediates signaling pathways critical for heart rate regulation .
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KCNK2 Modulation: Increases potassium channel density, stabilizing membrane potential in cardiac cells .
Clinical and Experimental Relevance
The POP2 antibody serves as a tool for:
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Cardiovascular Research: Studying POPDC2’s role in arrhythmias or heart failure.
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Therapeutic Development: Identifying POPDC2 as a potential target for modulating cardiac ion channel function .
Data Tables
Table 2: Research Applications
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- A strong connection exists between GABA metabolism and the glyoxylate cycle during stress. PMID: 23518583
- AtGABA-T is functionally interchangeable with ScGABA-TKG for GABA growth, thermotolerance, and limiting production of reactive oxygen species (ROS), irrespective of its location in mitochondria or cytosol of yeast cells. However, AtGABA-T is about half as efficient in performing these functions compared to ScGABA-TKG. PMID: 23740823
- GABA-T deficiency during salt stress leads to root and hypocotyl developmental defects and alterations in cell wall composition. PMID: 23148892
- The GABA transaminase deficient mutant pop2-1 exhibits hypersensitivity to NaCl. PMID: 20122158
- The isolation and characterization of the her1 mutant (encoding GABA-TP) revealed alterations in a specific response to E-2-hexenal. This study demonstrates that the mutation in GABA-TP leads to an accumulation of high GABA levels in her1 plants. PMID: 17971036
- Data suggests that POP2 mutants suppress the phenotype of succinic semialdehyde dehydrogenase (ssadh) mutants in Arabidopsis. PMID: 18846220
- GABA-T possesses both pyruvate- and glyoxylate-dependent activities. PMID: 19264755
Q&A
Here’s a structured FAQ collection for researchers working with POP2 (POPDC2) antibodies, synthesized from peer-reviewed methodologies and technical guidelines:
Advanced Experimental Design
How do I resolve discrepancies in POP2 expression data across studies?
Common pitfalls and solutions:
What controls are essential for flow cytometry experiments using POP2 antibodies?
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Fluorescence-minus-one (FMO) controls: Identify spillover between channels .
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Voltration: Optimize PMT voltages to avoid signal saturation (e.g., peak 2 method) .
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Biological controls: Compare wild-type vs. POP2-deficient cardiomyocytes .
Methodological Best Practices
How do I titrate a POP2 antibody for flow cytometry?
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Prepare serial dilutions (1:50 to 1:800) in PBS/1% BSA.
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Stain POP2-positive cells (e.g., primary cardiomyocytes).
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Select the dilution with the highest SI (typically 1:200 for ab224121 in IHC) .
Can POP2 antibodies be used in multiplex assays with other cardiac markers?
Yes, but prioritize spectral overlap resolution:
| Marker | Fluorophore | Laser Compatibility |
|---|---|---|
| POP2 (ab224121) | AF488 | 488 nm |
| Troponin T | PE-Cy7 | 561 nm |
| Connexin 43 | BV421 | 405 nm |
Source: Panel design principles from .
Troubleshooting Data Contradictions
Why does my POP2 antibody show non-specific binding in WB?
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Cause: Residual protein aggregates or incomplete blocking.
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Solution: Pre-clear lysates with protein A/G beads and use 5% non-fat milk + 0.1% Tween-20 .
