GAUT8 Antibody
Description
GAUT8 Protein Overview
GAUT8 (Galacturonosyltransferase 8) belongs to the CAZy GT8 family and is involved in synthesizing homogalacturonan (HG), a major pectin component. Key findings include:
-
Role in Cell Wall Integrity: GAUT8 is essential for cell adhesion and wall integrity, as gaut8 mutants exhibit reduced galacturonic acid (GalA) levels (25% reduction in leaves) and dwarfism .
-
Enzymatic Function: Biochemical studies confirm GAUT8 as a galacturonosyltransferase (GalAT), catalyzing the transfer of GalA residues to growing HG chains .
Research Applications of GAUT8 Antibodies
Though not explicitly detailed in the provided sources, antibodies targeting GAUT homologs (e.g., GAUT1) have been used in related studies. For example:
-
Immunoprecipitation: GAUT1 antibodies immunodepleted GalAT activity in Arabidopsis extracts, confirming its role in HG synthesis . Similar methodologies could theoretically apply to GAUT8.
-
Mutant Analysis: gaut8 mutants (e.g., qua1) show pleiotropic effects, including cell wall defects, which are often characterized using antibodies against cell wall components .
Key Phenotypic and Biochemical Data
Data from gaut8 mutants highlight its functional importance:
Methodological Insights
Studies on GAUT8 leverage techniques applicable to antibody-based research:
-
Immunoassays: GAUT1 antiserum was used to immunoprecipitate GalAT activity, a method transferable to GAUT8 studies .
-
Cell Wall Profiling: Monosaccharide composition analysis (e.g., HPAEC-PAD) and glycome profiling are critical for assessing GAUT8’s impact on pectin structure .
Future Directions
Product Specs
Target Background
- Studies suggest QUA1's role in chloroplast-dependent calcium signaling under salt and drought stress, with CAS potentially acting downstream. PMID: 28007965
- The QUA1 gene may have multiple functions in light signal transduction, influencing cell elongation and light-regulated gene expression. PMID: 27232492
- Research indicates a specific role for QUA1 in vascular tissue development within rapidly elongating inflorescence stems, supporting its involvement in pectin and hemicellulose cell wall synthesis. PMID: 16059719
- Perturbation of QUASIMODO 1-1 (QUA1) gene expression in calli altered homogalacturonan content and cell wall structure. PMID: 16263905
Q&A
Based on the provided search results, there is no available data specifically addressing GAUT8 antibodies. The materials extensively cover GAUT10-related research in plant cell wall biosynthesis , α-Gal antibodies , and diabetes-associated GAD/ZnT8 autoantibodies , but do not mention GAUT8. This discrepancy suggests either a nomenclature error (e.g., GAUT8 vs. GAUT10) or that GAUT8 antibodies represent an emerging research area not yet reflected in indexed literature.
Below are scientifically structured FAQs based on the closest related antibody research domains from the provided materials:
What experimental strategies resolve contradictions between α-Gal epitope detection methods in microbiota studies?
Conflicting results between monoclonal antibodies (e.g., 27H8 vs. BSI-B4) require:
How can researchers optimize multiplex autoantibody detection for autoimmune diabetes diagnostics?
The ZnT8/GAD65 chimeric protein assay demonstrates:
-
Epitope engineering: Fusion of immunodominant regions (ZnT8₃₂₋₃₆₉ + GAD₅₅₀₋₅₈₅) preserves conformational epitopes .
-
Platform comparison:
-
Clinical validation: Requires cohort studies comparing onset timelines in LADA vs. T1D patients .
What controls are essential when analyzing anti-α-Gal antibody responses in COVID-19 serology?
Critical controls include:
