GAUT10 Antibody

Biological Role of GAUT10

GAUT10 is a Golgi-localized enzyme critical for synthesizing pectin polysaccharides, particularly homogalacturonan (HG) and rhamnogalacturonan I (RG-I) . Key findings include:

Cell Wall Composition

• Loss of GAUT10 reduces galacturonic acid (GalA) and xylose in root cell walls, altering RG-I and HG polymer distribution .

• Mutants (gaut10-3) exhibit 15–20% lower GalA levels in stems and roots compared to wild-type plants .

Developmental Phenotypes

• gaut10 mutants display shortened roots due to reduced cell division in the root apical meristem (RAM) and impaired epidermal cell elongation .

• Double mutants (gaut10 gaut11) show enlarged stomatal complexes with defective guard cell dynamics .

Sucrose and Auxin Interactions

• The gaut10 short-root phenotype is sucrose-dependent and linked to peroxisome dysfunction .

• GAUT10 is post-transcriptionally regulated by auxin, influencing auxin metabolism and signaling pathways .

Research Applications of GAUT10 Antibody

The antibody has been instrumental in:

Protein Localization

• Confirming GAUT10’s Golgi localization via fluorescent tagging and colocalization with Golgi markers like GONST1 .

Mutant Characterization

• Validating gaut10 knockout lines (e.g., gaut10-1, gaut10-3) through Western blotting, showing absent or reduced protein levels .

Glycome Profiling

• Enabling ELISA-based epitope profiling to assess cell wall changes in mutants, revealing altered xylan and RG-I epitopes .

Key Studies Utilizing GAUT10 Antibody

Future Directions

Ongoing research employs the GAUT10 Antibody to explore:

• Hypocotyl elongation mechanisms in light/dark conditions .

• Cross-talk between pectin composition and stress responses (e.g., hydrogen peroxide signaling) .

• Role of GAUT10 paralogs (e.g., GAUT11) in redundant or distinct functions .