RACK1A Antibody
Description
Applications in Research
RACK1A Antibody is primarily used in Western blotting (WB) to detect RACK1A protein levels in plant tissues. It has also been employed in coimmunoprecipitation (CoIP) studies to identify RACK1A-interacting partners.
Key Applications
Reactivity and Specificity
The antibody exhibits high specificity for plant RACK1A isoforms, with confirmed reactivity in Arabidopsis thaliana and predicted cross-reactivity in Thellungiella salsuginea . It does not target human or animal RACK1 isoforms, distinguishing it from antibodies like RACK1 Antibody (ab129084) (Abcam) or RACK1 Antibody (AF3434) (R&D Systems), which are tailored for mammalian systems .
Species-Specific Reactivity
Plant Immunity and Stress Responses
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Role in ROS Production: Overexpression of RACK1A enhances ROS generation in rice, boosting resistance to Magnaporthe oryzae (rice blast) .
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Interaction with Rac1: RACK1A binds the N-terminal region of Rac1 (a GTPase) via its WD40 repeats, modulating immune responses .
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Regulation of Defense Genes: RACK1A RNAi suppresses PBZ1 (a pathogen-response gene) expression, impairing pathogen resistance .
Antiviral Translation Inhibition
RACK1A is exploited by viruses (e.g., herpes simplex virus) for internal ribosome entry site (IRES)-mediated translation. Small-molecule inhibitors targeting RACK1A's Y248 phosphorylation site disrupt viral mRNA translation, offering a broad antiviral strategy .
Leaf Senescence Regulation
RACK1A promotes ethylene-mediated leaf senescence by interacting with EIN3 (ETHYLENE INSENSITIVE 3) to inhibit miR164 transcription, thereby upregulating ORE1 (senescence-promoting gene) .
Translational Regulation
RACK1A binds ribosomes and regulates translation efficiency. Mutant RACK1A lacking ribosome-binding ability impairs cell cycle progression and global translation .
Table 2: Key Research Contributions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Two suppressor mutants of acl5-1, designated sac53-d and sac56-d, encode a receptor for activated C kinase 1 (RACK1) homolog and a ribosomal protein L4 (RPL4), respectively. PMID: 25625317
- A total of 97 proteins from pathways involved in dehydration, salt stress, ribosomes, and photosynthesis have been identified as potential interactors with RACK1A. PMID: 23435172
- RACK1 ensures the accumulation and processing of certain miRNA precursors, directly interacts with SERRATE, and is part of an AGO1 complex. PMID: 23941160
- RACK1A protein can form complexes with the gamma subunit of the signal transducing heterotrimeric G protein. PMID: 22068106
- RACK1 may be essential for the normal production of 60S and 80S ribosomes, and its function in these processes may be regulated by abscissic acid. [RACK1A] PMID: 21098678
- RACK1A (At1g18080) is involved in multiple signal transduction pathways. PMID: 16829549
- RACK1A (At1g18080) is positively regulated by other RACK1 genes. Loss of function results in defects in plant development. PMID: 18947417
- RACK1 genes act redundantly to negatively regulate abscisic acid responses during seed germination and early seedling development. PMID: 19584117
