GAMMACAL1 Antibody

Introduction to GAMMACAL1 Antibody

The GAMMACAL1 Antibody (Gamma Carbonic Anhydrase-Like 1 Antibody) is a monoclonal antibody targeting the CAL1 protein, a subunit of Complex I in the mitochondrial oxidative phosphorylation system. This antibody is primarily utilized in plant biology research, particularly in Arabidopsis thaliana, to study the structural and functional roles of Complex I in cellular respiration .

Biological Role

• Complex I Composition: CAL1 (AT5G63510) is one of at least 49 subunits in Arabidopsis Complex I, which catalyzes NADH-quinone oxidoreduction, a critical step in electron transport .

• Homology: CAL1 shares functional homology with Gamma Carbonic Anhydrase-Like 2 (CAL2/AT3G48680), another Complex I subunit, suggesting overlapping roles in mitochondrial metabolism .

Antibody Design

• Immunogen: Derived from the synthetic peptide sequence of AT5G63510 F4KAQ8, ensuring specificity to the CAL1 epitope .

• Cross-Reactivity: Recognizes CAL1 in Arabidopsis thaliana, Brassica rapa, and Brassica napus, making it a versatile tool for comparative plant studies .

Key Uses

Comparative Analysis with CAL2

Limitations and Future Directions

While the GAMMACAL1 Antibody is a critical reagent for plant mitochondrial studies, its utility in non-plant systems remains unexplored. Further research could expand its applications to other model organisms or explore therapeutic relevance, akin to monoclonal antibodies targeting human proteins .