GAMMACAL2 Antibody

Key Molecular Attributes

Functional Role of CAL2 in Mitochondrial Complex I

CAL2 is part of the 49-subunit Complex I in Arabidopsis, critical for electron transport and ATP synthesis. The antibody is designed to detect CAL2 for studying:

• Subunit interactions: CAL2’s role in stabilizing Complex I structure.

• Phylogenetic conservation: Cross-reactivity with Brassicaceae species suggests conserved function in plant mitochondria .

Key Findings

1. Epitope Specificity:

   • The synthetic peptide used for immunization shares 100% homology with CAL1, indicating potential cross-reactivity between CAL1 and CAL2 isoforms .

   • Immunoprecipitation studies in Arabidopsis suggest CAL2 is tightly associated with Complex I subunits, enabling its isolation for functional studies.

2. Cross-Species Utility:

   • Validated for Brassica rapa and Brassica napus, making it valuable for comparative studies in Brassicaceae .

Limitations and Considerations

• No Therapeutic Use: GAMMACAL2 Antibody is a research tool; no clinical applications are reported.

• Subunit Complexity: Complex I’s large size and subunit diversity may require additional antibodies for comprehensive analysis.