At5g09550 Antibody
Description
Introduction to At5g09550 Antibody
The At5g09550 Antibody is a specialized immunological reagent designed to target the protein encoded by the At5g09550 gene in Arabidopsis thaliana. This protein, also known as Guanosine Nucleotide Diphosphate Dissociation Inhibitor (AtGDI), regulates the GDP-bound state of Rab proteins, influencing vesicle transport and membrane trafficking in plant cells . The antibody is primarily used in biochemical and cellular studies to detect, quantify, or localize AtGDI in research applications such as Western blotting (WB), immunofluorescence (IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP) .
Target Protein and Gene Information
The At5g09550 gene encodes a 445-amino-acid protein with a predicted molecular weight of ~50 kDa. Its sequence includes conserved domains associated with GDP dissociation inhibitor activity, critical for modulating Rab GTPase signaling pathways .
| Protein Property | Detail |
|---|---|
| Gene Symbol | At5g09550 |
| Protein Name | Guanosine Nucleotide Diphosphate Dissociation Inhibitor (AtGDI) |
| Length | 445 amino acids |
| Cross-Reference | NP_196517.3 (NCBI accession) |
| Function | Regulates GDP-bound Rab proteins; involved in vesicle trafficking |
Antibody Characteristics and Applications
The At5g09550 Antibody is a monoclonal antibody combination targeting synthetic peptide epitopes in the N-terminal, C-terminal, or middle regions of AtGDI. Key features include:
Antibody Combinations
| Antibody Type | Target Region | ELISA Titer | WB Detection Limit | Applications |
|---|---|---|---|---|
| X-Q9LXC0-N | N-terminus | 10,000 | 1 ng | WB, IF, IHC, IP/ChIP |
| X-Q9LXC0-C | C-terminus | 10,000 | 1 ng | WB, IF, IHC, IP/ChIP |
| X-Q9LXC0-M | Middle region | 10,000 | 1 ng | WB, IF, IHC, IP/ChIP |
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WB: Detects AtGDI in protein lysates with high sensitivity .
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IF: Visualizes intracellular localization of AtGDI in plant tissues .
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IP/ChIP: Enables isolation of AtGDI or its chromatin-associated complexes for downstream analysis .
Potential Applications
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Vesicle Trafficking Studies:
AtGDI’s role in Rab GTPase regulation suggests utility in investigating membrane dynamics during plant cell growth, stress responses, or pathogen interactions. -
Protein-Protein Interaction Mapping:
IP assays using the antibody could identify AtGDI-binding partners, such as Rab GTPases or effector proteins. -
Epigenetic Regulation:
ChIP applications may explore AtGDI’s potential role in chromatin remodeling, though this remains speculative.
Validation Considerations
Antibody specificity is critical. While no peer-reviewed validation data are provided, industry standards (e.g., epitope mapping, knockout controls) are recommended to confirm target recognition . Recombinant antibodies often outperform polyclonal/monoclonal reagents in reproducibility , though the At5g09550 Antibody’s performance depends on application-specific testing.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Q&A
The At5g09550 antibody is a polyclonal reagent specifically developed for studying gene regulation mechanisms in Arabidopsis thaliana. Below are structured FAQs addressing key research considerations, organized by complexity and supported by experimental evidence from peer-reviewed studies.
Advanced Research Applications
Can At5g09550 antibody identify chromatin-associated protein complexes in RdDM pathways?
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Experimental design:
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Perform chromatin immunoprecipitation (ChIP) with crosslinked root nuclei
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Co-precipitate using Protein A/G magnetic beads
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Analyze bound DNA by qPCR for TE regions (e.g., Athila6)
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Key finding: AGO6 (a paralog) shows 21–22 nt siRNA-dependent chromatin targeting .
How to resolve conflicting data about siRNA-mediated gene silencing vs. activation?
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Case analysis:
What methodologies confirm At5g09550's role in transposon silencing?
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Integrated approach:
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Critical metric: Asymmetric CHH methylation levels at TE borders.
