At1g28660 Antibody

Target Protein: AT1G28660 (GDSL-Like Lipase/Acylhydrolase)

The At1g28660 gene encodes a member of the GDSL-motif esterase/acyltransferase/lipase family. This enzyme exhibits broad substrate specificity and may catalyze acyltransfer or hydrolase reactions with lipid and non-lipid substrates.

Enzyme Activity Studies

• Role in Lipid Metabolism: The GDSL-motif enzymes are implicated in lipid remodeling, cutin synthesis, and pathogen defense. The antibody could be used to probe the localization or abundance of At1g28660 in response to environmental stressors.

• Substrate Specificity: Western blotting with this antibody may help identify post-translational modifications or isoforms of the enzyme.

Comparative Protein Analysis

• Ortholog Identification: The antibody’s specificity for Arabidopsis allows cross-species comparisons to homologs in other plant species.

• Gene Knockout Studies: Used to validate gene deletion or overexpression constructs in functional genomics.

Limitations and Considerations

1. Application Scope: Currently validated only for ELISA and WB; other applications (e.g., immunoprecipitation) require further testing.

2. Cross-Reactivity: No data are available on potential cross-reactivity with non-Arabidopsis proteins.

3. Commercial Availability: Made-to-order with a lead time of 14–16 weeks, which may delay experimental timelines.