At1g28670 Antibody

Target Protein: AT1G28670 Gene Product

The AT1G28670 gene in Arabidopsis thaliana encodes a lipase protein involved in lipid metabolism and signaling. Key features include:

Antibody Development and Applications

The At1g28670 antibody was generated to investigate the spatial expression and functional roles of this lipase. Key research findings include:

Immunolocalization Studies

• The antibody detects the AT1G28670-encoded lipase in epidermal tissues, consistent with its role in cuticle formation .

• It has been used to confirm protein localization in chloroplasts under stress conditions, suggesting involvement in stress-responsive lipid remodeling .

Specificity and Validation

• Western blot analyses show a single band at ~45 kDa, matching the predicted molecular weight of the target protein .

• Cross-reactivity tests with other lipase isoforms (e.g., MIPS1, MIPS2) indicate partial recognition, necessitating careful experimental controls .

Role in Plant Defense

• AT1G28670 lipase activity is linked to pathogen response pathways, with knockout mutants showing increased susceptibility to fungal infections .

• Antibody-based assays revealed elevated protein levels during jasmonic acid signaling, implicating it in induced systemic resistance .

Technical Considerations

• False Positives: Like many plant antibodies, nonspecific binding to epitopes in related GDSL-motif proteins has been observed .

• Validation: Competitive ELISA and knockout mutant controls are recommended to confirm specificity .

Comparative Data

The table below summarizes key studies utilizing the At1g28670 antibody:

Limitations and Future Directions

• Specificity Challenges: Cross-reactivity with MIPS isoforms limits utility in complex tissue extracts .

• Functional Gaps: The exact catalytic mechanism and substrate preferences remain uncharacterized.

Recent advances in CRISPR-based tagging and nanobody engineering could address these limitations, enabling higher-resolution studies .