At1g29670 Antibody

Target Protein Overview

The AT1G29670 locus encodes a GDSL-motif lipase/acylhydrolase with predicted roles in:

• Lipid metabolism: Hydrolysis of ester bonds in lipids

• Cell wall dynamics: Participation in cell wall polysaccharide modifications under environmental stress

• Stress adaptation: Differential expression during sub-optimal growth conditions (e.g., temperature fluctuations)

Cell Wall Adaptation Studies

The antibody has been used to investigate cell wall protein (CWP) dynamics:

• Temperature stress: AT1G29670 protein levels increase in Arabidopsis floral stems grown at 15°C vs. 22°C, suggesting a role in cold adaptation .

• Subcellular localization: Predicted apoplastic activity, with potential involvement in lipid-derived signaling during pathogen defense .

Lipid Signaling Pathways

Studies link GDSL lipases to:

• Phosphatidic acid (PA) production: PA accumulation activates ROS/SA defense pathways in transgenic rapeseed overexpressing homologous GDSL enzymes .

• Guard cell regulation: Related GDSL lipases modulate stomatal dynamics via lipid hydrolysis in Arabidopsis .

Experimental Validation

• Western blot protocols: Optimized using 12% SDS-PAGE, PVDF membranes, and 1:1,000 antibody dilution .

• Housekeeping gene utility: AT1G29670 mRNA serves as a stable reference in RNAseq studies of auxin-modulated xyloglucan remodeling .

Functional Insights from Mutant Studies

While direct AT1G29670 knockout data is limited, related GDSL lipases show:

• Enhanced pathogen resistance: Overexpression of AtGDSL1 increases PA and SA levels, boosting Sclerotinia resistance in rapeseed .

• Reduced JA signaling: GDSL-mediated SA accumulation may antagonize jasmonic acid pathways .

Future Directions

• Substrate specificity: Biochemical characterization of recombinant AT1G29670 protein .

• Stress phenotyping: Functional analysis in AT1G29670 knockout/overexpression lines under biotic/abiotic stress.