At1g09390 Antibody
Description
Research Context and Target Protein
The At1g09390 gene encodes a GDSL-motif esterase/acyltransferase/lipase, part of an enzyme superfamily with broad substrate specificity . These enzymes catalyze reactions such as acyltransfer or hydrolysis in lipid and non-lipid pathways, though the precise biological function of At1g09390 remains under investigation.
Key Functional Insights:
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Enzyme Activity: Likely involved in acyl lipid metabolism or secondary metabolite biosynthesis.
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Subcellular Localization: Predicted to be secreted, based on protein structure .
Applications in Research
The antibody is validated for:
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Western Blotting: Detects the At1g09390 protein in Arabidopsis tissue extracts.
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ELISA: Quantifies protein levels in purified samples or lysates.
Example Workflow:
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Sample Preparation: Homogenize Arabidopsis tissues in lysis buffer.
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SDS-PAGE/WB: Resolve proteins, transfer to membrane, and probe with the antibody.
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Signal Detection: Use HRP-conjugated secondary antibodies for chemiluminescent detection.
Note: Cross-reactivity with non-Arabidopsis species has not been reported, but rigorous validation is recommended for novel applications .
Availability and Considerations
The antibody is available in custom quantities (2ml or 0.1ml vials) through Cusabio’s catalog . Key considerations include:
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Order Lead Time: 14–16 weeks due to made-to-order production.
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Handling: Requires storage at -20°C or -80°C to maintain stability.
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Limitations: Not validated for immunohistochemistry (IHC) or immunoprecipitation (IP).
Comparative Analysis with Related Antibodies
Cusabio offers a suite of Arabidopsis-specific antibodies, including:
| Antibody/Target | Product Code | Uniprot ID | Applications |
|---|---|---|---|
| At1g09390 Antibody | CSB-PA525693XA01DOA | O80522 | ELISA, WB |
| At5g45950 Antibody | CSB-PA880756XA01DOA | Q9FJ41 | ELISA, WB |
| At1g28580 Antibody | CSB-PA861896XA01DOA | Q9FXJ2 | ELISA, WB |
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
Q&A
FAQs for At1g09390 Antibody in Academic Research
Advanced Research Questions
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How to resolve contradictory data between ELISA and Western blot results?
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Root causes and solutions:
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Epitope masking in WB: Use antigen retrieval via heat-mediated SDS treatment or trypsinization.
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Non-specific binding in ELISA: Include a negative control antigen (e.g., GST-tagged irrelevant protein) to assess off-target binding .
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Quantitative discrepancies: Normalize signals using housekeeping proteins (e.g., actin) in WB or spike-in controls in ELISA.
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Can At1g09390 antibody be used for co-immunoprecipitation (Co-IP) in protein interaction studies?
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How to design a study investigating At1g09390’s role in stress response pathways?
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Integrated workflow:
Technique Key Insight CRISPR-Cas9 mutants Phenotypic analysis of gene loss Phospho-specific probes Activation status of signaling pathways Single-cell RNA-seq Cell-type-specific expression -
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What computational tools aid in epitope mapping for At1g09390 antibody?
Methodological Best Practices
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Storage: Aliquot and store at -80°C in 50% glycerol to prevent freeze-thaw degradation .
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Reproducibility: Use batch-specific validation for long-term studies (e.g., compare lot numbers via WB).
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Multiplexing: Pair with antibodies against compartment markers (e.g., nuclear/chloroplast) for subcellular localization studies.
