At1g54020 Antibody

Definition and Target

The At1g54020 antibody is a polyclonal antibody raised against the protein encoded by the At1g54020 gene in Arabidopsis thaliana. This gene is annotated as GLIP1 (GDSL Lipase 1), which belongs to the GDSL lipase/esterase family involved in lipid metabolism and stress responses . The antibody detects the target protein in techniques such as Western blot (WB) and immunohistochemistry (IHC).

Research Context

The At1g54020 gene product interacts with gibberellin signaling pathways. A study by Crocco et al. (2015) demonstrated that RGA (Repressor of GA1-3), a DELLA protein in Arabidopsis, is critical for regulating xylem fiber differentiation . While this work primarily used anti-RGA antibodies, it highlights the importance of antibodies in studying plant developmental pathways where At1g54020 may play a role.

Validation Challenges

Antibody specificity is a known issue in plant research. For example, commercial antibodies against mammalian proteins like angiotensin receptors often show nonspecific binding in Western blots . While no direct validation data for At1g54020 antibodies are publicly available, researchers are advised to:

• Use knockout mutants as negative controls.

• Cross-validate with orthogonal methods (e.g., mass spectrometry).

Functional Insights

Proteomic studies of Arabidopsis vacuoles identified 163 tonoplast proteins, including transporters and hydrolases . Though At1g54020 was not explicitly listed, lipid-modifying enzymes like GLIP1 are critical for membrane dynamics, suggesting potential applications for this antibody in studying subcellular localization.

Future Directions

Emerging antibody characterization platforms, such as peptide microarrays , could improve validation workflows for plant-specific antibodies. Collaborative databases documenting antibody performance (e.g., signal intensity, cross-reactivity) would enhance reproducibility in plant biology research.