MVP1 Antibody
Description
Introduction to MVP1 and its Role in Biological Systems
MVP1 (Major Vault Protein 1), also known as LRP (Lung Resistance Protein), is a 110 kDa structural component of vault complexes—large ribonucleoprotein particles involved in nucleo-cytoplasmic transport, chemoresistance, and signaling regulation . MVP1 is overexpressed in multidrug-resistant cancers, including papillary thyroid cancer (PTC), breast, liver, and colon cancers, where it correlates with poor prognosis . Antibodies targeting MVP1 are critical tools for studying its role in oncogenesis, immune modulation, and therapeutic resistance.
Applications and Detection Methods Using MVP1 Antibodies
MVP1 antibodies are utilized in diverse experimental workflows:
| Application | Method | Key Antibodies | Reactivity |
|---|---|---|---|
| Western Blot (WB) | Protein detection | Abcam ab97311, Proteintech 16478-1-AP | Human, Mouse, Rat |
| Immunohistochemistry (IHC) | Tissue localization | ab97311, 16478-1-AP | Human, Mouse |
| Immunofluorescence (ICC/IF) | Cellular localization | ab97311, 16478-1-AP | Human, Mouse |
| Immunoprecipitation (IP) | Protein interaction studies | 16478-1-AP | Human, Mouse |
Notable Antibodies:
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Abcam ab97311: Rabbit polyclonal antibody validated in WB, IHC, and ICC/IF for human/mouse samples, with a predicted band size of 99 kDa .
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Proteintech 16478-1-AP: Rabbit polyclonal antibody reactive with human, mouse, and rat samples, tested in WB, IHC, IF, and IP .
Key Research Findings and Clinical Relevance
MVP1’s role in cancer progression and immune modulation has been extensively studied:
Clinical Implications:
MVP1 serves as a biomarker for chemoresistance and immune evasion. Its overexpression in cancers like PTC is linked to BRAF V600E mutations, suggesting therapeutic targeting potential .
Mechanistic Insights from Functional Studies
MVP1’s oncogenic roles are mediated through:
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Pathway Activation: Enhances PI3K/AKT/mTOR and MAPK/ERK signaling, driving proliferation and survival .
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Endosomal Recycling: In yeast, MVP1 (Mvp1) deforms endosomal membranes and recruits Vps1 (dynamin-like GTPase) to scission recycling tubules, a process conserved in humans via SNX8 .
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Immune Microenvironment Modulation: High MVP1 expression correlates with increased CD8+ T cell infiltration but poor clinical outcomes, indicating complex immune regulation .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research indicates that ERMO3/MVP1/GOLD36 is essential for preventing abnormal aggregation of the ER and other organelles, contributing to the maintenance of normal ER morphology. This function is coordinated with NAI1. PMID: 23155454
- A missense mutation within the gold36 locus (At1g54030), which encodes a small endomembrane protein, results in organizational defects within the ER and aberrant protein trafficking within the plant secretory pathway. PMID: 20626647
