At1g74460 Antibody
Description
Gene Function and Biological Relevance
The AT1G74460 gene in Arabidopsis thaliana encodes a GDSL-motif esterase/acyltransferase/lipase, part of a superfamily with broad substrate specificity. These enzymes catalyze acyltransfer or hydrolase reactions, potentially involving lipid and non-lipid substrates .
Key Functional Insights:
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Enzyme Group: GDSL-motif enzymes are implicated in plant defense, stress responses, and secondary metabolism.
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Substrate Specificity: Broad, including lipids, esters, and other organic compounds.
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Localization: Likely cytosolic or membrane-associated, given the enzyme’s role in metabolic pathways.
Validation and Specificity Considerations
While the At1G74460 Antibody is designed for specificity, commercial antibodies often face challenges in validation. Studies on similar Arabidopsis antibodies highlight critical validation steps:
Common Challenges in Antibody Specificity:
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Cross-Reactivity: Potential binding to homologous GDSL-motif proteins.
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Non-Specific Bands: Western blot artifacts due to epitope similarity.
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Negative Controls: Use of at1g74460 knockout mutants to confirm target absence.
Example Validation Protocol:
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Western Blot: Resolve proteins from Arabidopsis tissue lysates, probe with At1G74460 Antibody.
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Immunoprecipitation: Verify co-purification of the target protein.
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Functional Assays: Correlate antibody binding with enzymatic activity inhibition.
Refer to antibody validation guidelines from studies on AT1 receptor antibodies .
Potential Applications in Research
The At1G74460 Antibody serves as a tool for investigating:
| Application | Experimental Approach |
|---|---|
| Protein Localization | Immunofluorescence in Arabidopsis tissues. |
| Enzyme Activity | Inhibition assays using purified protein. |
| Stress Response | Analysis of AT1G74460 expression under abiotic stress. |
| Metabolic Pathway | Co-IP with GDSL-enzyme interactors. |
