At2g30220 Antibody

Introduction to AT2G30220 Antibody

The AT2G30220 antibody targets the protein encoded by the Arabidopsis thaliana gene AT2G30220, which belongs to the GDSL-like Lipase/Acylhydrolase family. This enzyme is implicated in lipid metabolism, plant defense mechanisms, and stress responses . Antibodies against plant proteins like AT2G30220 are critical tools for studying localization, expression dynamics, and functional roles in plant biology.

Gene and Protein Characteristics

This protein is structurally characterized by a conserved GDSL motif, which facilitates its enzymatic activity in lipid metabolism pathways .

Key Steps in Antibody Production

1. Antigen Design: Peptides corresponding to unique epitopes in the AT2G30220 protein sequence are synthesized.

2. Immunization: Host animals (e.g., rabbits) are immunized to generate polyclonal antibodies .

3. Purification: Affinity chromatography isolates antibodies specific to AT2G30220 .

4. Validation:

   • Specificity: Western blotting against Arabidopsis wild-type and at2g30220 knockout mutants .

   • Cross-Reactivity: Testing against homologous proteins in related plant species .

Performance Metrics

Functional Studies

• Lipid Metabolism: AT2G30220 antibody has been used to track lipid droplet dynamics during pathogen infection .

• Stress Responses: Elevated protein levels under drought and salinity stress, as quantified by immunoblotting .

Comparative Analysis

Challenges and Limitations

• Cross-Reactivity: Some commercial plant antibodies show nonspecific binding to unrelated epitopes . For AT2G30220, rigorous validation with knockout lines is essential.

• Low Abundance: Native protein levels are often below detection thresholds, requiring signal amplification methods .

Future Directions

• Structural Studies: Cryo-EM or X-ray crystallography to resolve AT2G30220-lipid complexes .

• Therapeutic Potential: Engineered plant-derived antibodies for agricultural biotechnology .