At2g30310 Antibody

Advanced Research Questions

How to resolve contradictory data in protein interaction studies?

• Case Example: If yeast-two-hybrid shows interactions unconfirmed by co-IP:

  • Test alternative extraction buffers (e.g., +1% CHAPS for membrane proteins)

  • Validate using orthogonal methods like BiFC or FRET-FLIM

  • Consider temporal expression differences via qRT-PCR across growth stages

How to optimize protocols for low-abundance targets?

• Combine signal amplification methods:

  • Tyramide-based TSA for microscopy

  • Sequential IP-LC/MS for proteomics

• Validate using serial dilutions of recombinant protein spiked into wild-type extracts

What systems validate antibody function in planta?

Data Interpretation Guidelines

7. Quantitative analysis standards for developmental studies:

• Normalize to internal reference proteins (e.g., AT5G46630, a constitutively expressed control )

• Statistical reporting: Include Cohen’s d effect size for mutant vs. wild-type comparisons