At2g31550 Antibody

Gene Function

• At2g31550: Classified under the GDSL-motif lipase/hydrolase family, which is associated with:

  • Hydrolysis of ester bonds in lipids .

  • Plant defense mechanisms and stress adaptation.

  • Cell membrane remodeling during growth and development.

Protein Structure

• The protein contains a conserved GDSL motif, critical for enzymatic activity.

• Predicted molecular weight: ~45 kDa (exact value not provided in sources).

Research Applications

• Functional Studies: GDSL lipases in Arabidopsis are implicated in cutin synthesis and pathogen resistance .

• Epigenetic Research: At2g31550 was analyzed in a transcriptome study of autopolyploid Arabidopsis, though no direct link to antibody use was noted .

Validation and Specificity Concerns

• Cross-Reactivity: No data on cross-reactivity with other proteins or species is available.

• Validation Gaps:

  • Source highlights widespread issues with nonspecificity in commercial antibodies targeting plant proteins, underscoring the need for independent validation.

  • No Western blot or immunoprecipitation data confirming target specificity is provided for this antibody.

Critical Gaps and Future Directions

• Functional Data: No studies demonstrate the antibody’s utility in vivo or in vitro.

• Comparative Analyses: Lack of comparisons with knockout mutants to confirm specificity.

• Therapeutic/Agricultural Potential: Unclear due to insufficient research.