At2g38180 Antibody
Description
Introduction to At2g38180 Antibody
The At2g38180 Antibody is a polyclonal immunoglobulin (IgG) raised in rabbits, specifically designed to target the At2g38180 protein in Arabidopsis thaliana (mouse-ear cress). This antibody is critical for studying the functional role of At2g38180 in plant biology, particularly in gene expression, cellular processes, or environmental responses. Its development and characterization align with modern antibody validation standards, emphasizing specificity and reproducibility in experimental workflows .
Assay and Gene Information
The At2g38180 Antibody is linked to assays targeting the AT2G38180 gene in Arabidopsis. Key details include:
Table 1: Assay and Gene Details
| Parameter | Value |
|---|---|
| Gene Symbol | AT2G38180 |
| Synonyms | F16M14.11, F16M14_11 |
| Chromosome | Chromosome 2 |
| Interrogated Sequence | RefSeq (NM_129374.4), GenBank (BT021087.1, BT021116.1) |
| Amplicon Length | 78 bp (RefSeq), 78 bp (GenBank) |
| Exon Boundaries | Spanning exons 4–5 |
| Probe Application | Detects genomic DNA in Arabidopsis thaliana |
The antibody’s specificity is validated for genomic DNA detection, with amplicon sequences aligning to exons 4–5 of the target gene .
Applications and Usage
The At2g38180 Antibody is primarily used in basic research to study:
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Gene Expression: Quantification of At2g38180 mRNA/protein levels via ELISA or WB.
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Protein Localization: WB analysis to assess subcellular distribution in plant tissues.
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Functional Studies: Knockout (KO) or overexpression models to dissect At2g38180’s role in stress responses or development.
While no peer-reviewed studies explicitly cite this antibody, its design aligns with standard practices for plant protein analysis . Researchers should validate its performance in their experimental context, particularly for novel applications.
Best Practices for Antibody Validation
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Target Binding: Confirm binding specificity using KO cell lines or recombinant antigens .
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Cross-Reactivity: Test against non-target proteins (e.g., homologs in other species).
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Assay Optimization: Validate dilution ranges and blocking conditions for ELISA/WB.
General Challenges
Product Specs
Composition: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Q&A
Here’s a structured collection of FAQs for researchers working with the At2g38180 antibody, synthesized from peer-reviewed methodologies and experimental insights:
Advanced Research Questions
How to resolve conflicting data between ELISA and pull-down assays for At2g38180 interaction studies?
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Analysis framework:
Assay Strengths Limitations ELISA High sensitivity May detect non-physiological interactions Pull-down Captures native complexes Low abundance targets may be missed -
Resolution: Use surface plasmon resonance (SPR) to measure binding kinetics (ka/kd) and confirm affinity .
What strategies improve antibody efficacy in in planta functional studies?
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Engineering approaches:
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Validation: Test engineered variants in Arabidopsis knockout-complementation assays .
How to identify critical quality attributes (CQAs) affecting At2g38180 antibody-antigen binding?
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Workflow:
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Critical modifications: Prioritize residues in complementarity-determining regions (CDRs) with >2-fold abundance in unbound vs. bound fractions (p<0.01) .
Methodological Comparisons
Table 1. Antibody validation techniques for plant studies
| Method | Resolution | Throughput | Key application |
|---|---|---|---|
| IP-MS | 1–5 kDa | Low | Specificity confirmation |
| SEC + SDS-PAGE | 10–100 kDa | Medium | Complex stability analysis |
| SPR | Single residue | High | Binding kinetics quantification |
