At3g14220 Antibody

Introduction to At3g14220 Antibody

The At3g14220 Antibody is a polyclonal antibody raised against the protein encoded by the At3g14220 gene in Arabidopsis thaliana (thale cress). This antibody is used primarily in plant molecular biology research to study protein localization, function, and interactions. While direct experimental data on its applications remain limited, its availability as a commercial product underscores its utility in plant proteomics and functional genomics .

Gene Annotation and Functional Role

The At3g14220 gene encodes a myrosinase-associated protein-like (MAP-like) protein. Myrosinase-associated proteins are typically involved in glucosinolate metabolism, a critical defense mechanism in plants against herbivores and pathogens. These proteins stabilize myrosinase enzymes, which hydrolyze glucosinolates into toxic isothiocyanates .

Key Gene Features:

Note: Subcellular localization data inferred from Arabidopsis protein databases .

Research Applications and Potential Use Cases

While no peer-reviewed studies explicitly describe At3g14220 Antibody applications, its design aligns with common uses in plant biology:

1. Protein Localization

   • Immunofluorescence or immunoelectron microscopy to map the subcellular distribution of At3g14220 in plant tissues.

   • Example: Confirming membrane localization (M) or histone-associated (H) localization patterns .

2. Functional Studies

   • Co-immunoprecipitation to identify interacting proteins, such as myrosinase or glucosinolate biosynthesis enzymes.

   • Enzyme activity assays to assess the role of At3g14220 in glucosinolate metabolism.

3. Genetic Knockout Validation

   • Western blot detection of At3g14220 protein in wild-type vs. knockout mutants to confirm gene silencing .

Table 2: Hypothetical Research Workflow

Limitations and Future Directions

Current limitations include:

• Sparse Functional Data: No published studies directly validate At3g14220’s role in glucosinolate metabolism or stress responses.

• Species Specificity: Limited cross-reactivity data with other plant species.

Future research could focus on:

• CRISPR-Cas9 Knockouts: Phenotyping mutants to link At3g14220 to biochemical pathways.

• Omics Integration: Proteomic profiling of At3g14220 interactomes using mass spectrometry .