At4g30140 Antibody
Description
Target Protein Overview
The At4g30140 Antibody recognizes CDEF1, a member of the GDSL lipase/esterase family . CDEF1 is implicated in cuticle destruction, as suggested by its classification as a candidate gene encoding a cutinase. The GDSL family is characterized by a conserved glycosyl-aspartate-serine-leucine (GDSL) motif, which enables enzymatic activity in lipid metabolism and cuticle degradation .
Research Applications and Techniques
The At4g30140 Antibody is primarily used in plant biology research, particularly for studying:
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Cuticle development and degradation
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Enzyme localization and activity
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Protein interactions
Common techniques include:
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Western blotting: To detect CDEF1 protein expression levels.
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Immunoprecipitation: To isolate CDEF1 for functional studies.
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Immunofluorescence: To visualize CDEF1 localization in plant tissues.
Research Findings and Key Studies
The At4g30140 Antibody has enabled critical insights into CDEF1’s role in Arabidopsis:
Key Study: CDEF1 as a Cutinase
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PMID 19996150: Identified AT4G30140 as a candidate gene encoding a cutinase involved in cuticle destruction.
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Functional Role: CDEF1 hydrolyzes cutin, a key component of the plant cuticle, suggesting its involvement in defense mechanisms or developmental processes.
Database Links and Orthologs
The AT4G30140 gene is annotated in multiple databases:
