At5g03590 Antibody

Overview of At5g03590 Antibody

The At5g03590 antibody is a polyclonal antibody designed to detect the protein product of the At5g03590 gene in Arabidopsis thaliana. This gene encodes a protein with the UniProt identifier Q9LZS9, though its precise biochemical function remains under investigation. The antibody is used primarily in immunoblotting (Western blot), immunohistochemistry (IHC), and immunofluorescence (IF) applications .

Role in Plant Biology

While the exact biological role of the At5g03590 protein is not fully characterized, antibodies like this are critical for:

• Protein Localization Studies: Mapping subcellular distribution in plant tissues .

• Expression Profiling: Assessing protein levels under varying environmental or genetic conditions .

• Interaction Networks: Identifying binding partners via co-immunoprecipitation (Co-IP) .

Validation and Quality Control

The antibody’s specificity is confirmed through:

• Knockdown/Knockout Validation: Loss of signal in At5g03590-deficient plant lines .

• Cross-Reactivity Testing: No observed reactivity with unrelated Arabidopsis proteins .

• Batch Consistency: Rigorous quality checks ensure lot-to-lot reproducibility .

Comparison with Related Antibodies

The table below contrasts At5g03590 with other Arabidopsis-targeting antibodies from the same supplier :

Availability and Usage Notes

• Storage: Stable at -20°C for long-term storage; avoid freeze-thaw cycles .

• Recommended Dilutions:

  • Western Blot: 1:500–1:2000

  • Immunohistochemistry: 1:50–1:200

Limitations and Future Directions

• Unknown Protein Function: The At5g03590 protein’s role in plant physiology requires further study.

• Species Restriction: Reactivity is limited to Arabidopsis thaliana; cross-species applications are untested .