At5g18430 Antibody

Introduction to At5g18430 Antibody

The At5g18430 antibody is a specialized reagent targeting the GDSL esterase/lipase protein encoded by the At5g18430 gene in Arabidopsis thaliana. This antibody is critical for studying gene expression, protein localization, and functional roles in plant physiology, particularly in stomatal guard cell (GC) regulation. Below is a structured breakdown of its development, applications, and research findings.

Gene At5g18430: Functional and Molecular Context

The At5g18430 gene encodes a GDSL-motif esterase/lipase, part of a superfamily with broad enzymatic activity (e.g., acyltransferase, hydrolase reactions) .

Functional Insights:

• Guard Cell Specificity: Promoter GUS assays revealed strong GC-preferential expression, with minimal activity in non-GC tissues .

• Role in Stomatal Movement: Null mutants (at5g18430) exhibited impaired blue light (BL)-induced stomatal opening, linking the gene to light signal transduction .

Stomatal Regulation Studies

At5g18430 is implicated in stomatal movement, a critical process for gas exchange and drought tolerance. Key findings include:

• Null Mutant Phenotype: at5g18430 mutants showed reduced stomatal aperture under BL illumination, indicating a role in light-responsive signaling .

• Promoter Activity: The 3-kb promoter region of At5g18430 drives GC-specific expression, surpassing other GC-preferential genes in selectivity .

Antibody Utility in Immunological Assays

The antibody is validated for:

• Western Blot (WB): Detection of At5g18430 protein in plant extracts, with sensitivity down to 1 ng .

• ELISA: Quantitative analysis of protein abundance in GC-enriched tissues .

Availability and Purchasing Options

Antibodies are commercially available through providers like Abmart, with options for:

• Single Combinations: N, C, or M terminus-specific antibodies ($599 each) .

• Custom Projects: Tailored antibody development for specialized needs (e.g., family cross-reactivity) .

Significance and Future Directions

The At5g18430 antibody serves as a vital tool for dissecting GC biology, with implications for:

• Agricultural Biotechnology: Engineering drought-resistant crops via stomatal regulation.

• Basic Research: Elucidating GDSL esterase/lipase functions in lipid metabolism and stress responses.

Further studies could explore epitope mapping or antibody-based therapeutics for plant stress management.