GDV1 Antibody, FITC conjugated
Description
Introduction to GDV1 Antibody, FITC Conjugated
FITC (fluorescein isothiocyanate)-conjugated GDV1 antibodies would be specialized reagents designed to detect the GDV1 protein, a nuclear factor critical for sexual development in Plasmodium parasites . While no commercial GDV1-FITC antibody is explicitly documented in the search results, analogous FITC-conjugated antibodies (e.g., anti-GFP , anti-collagen ) suggest standard conjugation protocols and applications would apply.
Antibody Characteristics (Hypothetical)
Based on established FITC conjugation practices , key features would likely include:
Immunofluorescence (IF)
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Nuclear Localization: GDV1 regulates heterochromatin dynamics via HP1 interaction . FITC labeling would enable visualization of GDV1’s punctate nuclear distribution, akin to GFP-tagged GDV1 imaging .
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Protocol: Dilute 1:500 in PBS/10% FBS, incubate 1 hr at RT .
Western Blotting (WB)
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Detection: Expected to identify GDV1 at ~50–70 kDa (size varies by species). Cross-reactivity testing would be essential, as seen with anti-GFP validation .
Flow Cytometry
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Cell Cycle Analysis: GDV1’s role in sexual development could be studied in parasite cultures using FITC signal intensity .
Research Findings (Inferred from GDV1 Biology)
GDV1 truncation mutants (e.g., GDV1Δ39) show diffuse nuclear localization compared to wild-type , which a FITC-conjugated antibody could quantify. Key functional insights include:
| GDV1 Feature | Biological Impact | Detection Method |
|---|---|---|
| Full-length C-terminus | HP1 binding, heterochromatin dissociation | IF with FITC conjugate |
| Δ39 truncation | Loss of nuclear puncta | Reduced FITC signal intensity |
Comparison with Similar FITC-Conjugated Antibodies
| Antibody | Target | Host | Applications | Reference |
|---|---|---|---|---|
| Anti-GDV1 (hypothetical) | GDV1 | Rabbit | IF, WB, Flow | Inferred |
| Anti-GFP | GFP | Goat | WB, IF | |
| Anti-Collagen I | Collagen I | Rabbit | WB, IHC, Flow | |
| Anti-IFN-α | Mouse IFN-α | Rat | Flow, IHC |
Limitations and Considerations
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Specificity: Requires validation via knockout controls, as non-specific binding is common in FITC conjugates .
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Photostability: FITC fluorescence degrades under prolonged light exposure , necessitating shielded storage.
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Cross-Reactivity: Epitope mapping is critical; GDV1 homologs in other species may require separate validation .
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
