At5g13200 Antibody

Introduction to At5g13200 Antibody

At5g13200 Antibody refers to a specific immunological reagent targeting the protein encoded by the AT5G13200 gene in Arabidopsis thaliana (thale cress). This antibody is used in molecular biology and plant genetics research to study gene expression, protein localization, and functional interactions of the associated GRAM domain-containing protein . Its applications include investigating stress responses, phytohormone signaling, and transposable element (TE) interactions in plant cells .

Biological Function of AT5G13200

The AT5G13200 gene encodes a GRAM domain family protein, which is implicated in:

• Abiotic stress response: Activation by environmental stressors such as drought or salinity .

• Phytohormone regulation: Interaction with abscisic acid (ABA), a hormone critical for stress adaptation and seed dormancy .

• Reproductive development: Roles in seed germination and floral development .

A key feature of AT5G13200 is a retrotransposon (ATCOPIA78/ONSEN) inserted in its 3′-UTR, which promotes antisense transcription and epigenetic regulation .

Research Applications of At5g13200 Antibody

The antibody enables:

1. Protein Detection: Western blotting or immunoprecipitation to quantify AT5G13200 protein levels in plant tissues .

2. Gene Expression Analysis: Combined with qPCR to study transcriptional regulation under stress or TE insertion .

3. Subcellular Localization: Immunofluorescence microscopy to map protein distribution in cell membranes or organelles .

Example applications include:

• Investigating ABA-mediated drought tolerance in Arabidopsis .

• Analyzing TE-antigen interactions in seed dormancy regulation .

Key Research Findings

1. TE-Mediated Regulation:

   • The ATCOPIA78 insertion in AT5G13200’s 3′-UTR induces antisense transcription, generating non-coding RNAs that modulate epigenetic silencing .

   • Mutants (ibm2, edm2, suvh456) disrupt TE-driven transcription, reducing full-length mRNA and increasing truncated isoforms .

2. Stress Response Pathways:

   • AT5G13200 expression is upregulated by ABA and abiotic stress, suggesting a role in stress adaptation .

   • Antisense transcripts may compete with sense mRNA for translation, fine-tuning protein levels under stress .

Future Directions

• Epigenetic Studies: Investigating how TE insertions and antisense RNAs influence DNA methylation or histone modification at AT5G13200.

• Protein Interactions: Mapping GRAM domain partners to elucidate downstream signaling pathways.

• Agricultural Applications: Evaluating AT5G13200’s potential in breeding stress-resilient crops via CRISPR or transgenic approaches .