At5g23350 Antibody

Introduction to At5g23350 Antibody

The At5g23350 Antibody is a polyclonal antibody raised in rabbits, designed to target the At5g23350 gene product in Arabidopsis thaliana. This protein belongs to the GRAM domain family and is associated with abscisic acid (ABA)-responsive pathways, playing a role in plant stress responses such as drought or salt stress . The antibody is primarily used for research applications in molecular biology, including ELISA and Western Blot (WB), to study protein localization and expression under stress conditions .

Biological Context of At5g23350 Protein

The At5g23350 gene encodes a GRAM domain-containing protein implicated in ABA signaling, a critical pathway for drought and salt stress responses in plants . Key findings include:

• ABA-Responsive Expression: At5g23350 is transcriptionally upregulated in Arabidopsis tumors and under drought conditions, suggesting a role in stress adaptation .

• Functional Partnerships: The protein interacts with ABA-dependent phosphatases (e.g., ABI1, ABI2) and transcription factors (e.g., ABI4), which are critical for ABA-mediated stress protection .

• Stress-Induced Regulation: In salt-stressed nhx1 mutant plants (lacking a vacuolar Na+/H+ antiporter), At5g23350 showed a 1.08-fold increase in expression, highlighting its involvement in ion homeostasis .

Research Applications of At5g23350 Antibody

The antibody is utilized to probe the expression and localization of the At5g23350 protein in experimental systems:

Validation and Cross-Reactivity Considerations

While the antibody is validated for Arabidopsis, researchers should:

• Confirm Specificity: Use orthogonal methods (e.g., CRISPR knockouts, peptide blocking) to exclude off-target binding .

• Optimize Conditions: Adjust dilution ratios for WB/ELISA to ensure signal-to-noise ratio .

• Cross-Species Reactivity: Limited to Arabidopsis; not tested in other plant or animal models .