At5g23360 Antibody
Description
Introduction to At5g23360 Antibody
The At5g23360 antibody (Product Code: CSB-PA861854XA01DOA) is a monoclonal or polyclonal immunoglobulin produced to detect and quantify the AT5G23360 protein. This protein is linked to abscisic acid (ABA)-mediated responses and seed development in plants .
Target Protein Characteristics
The AT5G23360 gene encodes an ABA-responsive protein with roles in:
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Seed maturation: Regulation of desiccation tolerance and dormancy .
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Stress adaptation: Participation in drought and salinity response pathways .
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Developmental signaling: Interaction with transcription factors like ABI3/VP1, which modulate gene expression during stress .
Key Studies:
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Role in Conifer Stress Response:
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AT5G23360 homologs in Yellow cedar (Callitropsis nootkatensis) were shown to interact with ABI3, a transcription factor critical for ABA signaling during seed development and stress adaptation .
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Experimental knockdown of this protein disrupted ABA-mediated gene activation, impairing drought tolerance .
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ABA Biosynthesis Link:
Applications in Plant Biology
The At5g23360 antibody is utilized for:
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Western blotting: Protein expression profiling under ABA treatment.
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Immunohistochemistry: Localization studies in seed tissues and meristems.
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Protein interaction assays: Identification of binding partners in ABA signaling complexes.
AT5G23360 vs. Homologs in Other Species:
| Feature | AT5G23360 (A. thaliana) | Homolog in Yellow Cedar |
|---|---|---|
| Function | ABA signaling, seed development | Stress adaptation, ABA response |
| Expression | Seed-specific | Ubiquitous under stress |
| Interaction | ABI3/VP1 | ABI3-interacting protein |
Future Directions
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Mechanistic studies: Elucidate post-translational modifications of AT5G23360 under ABA treatment.
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Crop engineering: Explore overexpression of AT5G23360 to enhance stress tolerance in agronomically important species.
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Q&A
Here’s a structured FAQ addressing research applications of the AT5G23360 antibody, incorporating experimental design principles, data interpretation challenges, and methodological guidance based on current literature:
Advanced Research Questions
How to design experiments resolving contradictory data on AT5g23360’s role in RdDM?
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Contradiction example: Discrepancies in AGO4 colocalization between hsp20 mutants and wild-type .
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Approach:
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Combine ChIP-seq (AT5g23360 antibody) with RNA FISH to correlate DNA methylation and non-coding RNA expression.
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Use fluorescence lifetime imaging microscopy (FLIM) to assess protein-protein interaction dynamics.
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Validate with genetic complementation assays in at5g23360 mutants.
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What statistical methods are optimal for analyzing AT5g23360-dependent methylation patterns?
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Workflow:
Data Tables
Table 1: AT5g23360 Antibody Performance in Mutant Backgrounds
| Mutant Line | AGO4 Colocalization (%) | RdDM Efficiency (Δ methylation) | Source |
|---|---|---|---|
| Wild-type | 92 ± 3.1 | Baseline (0%) | |
| hsp20 | 64 ± 5.8* | +18%* | |
| hen1 | 88 ± 2.4 | -29%* |
Table 2: Common Technical Artifacts and Solutions
Methodological Recommendations
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For Co-IP studies: Include MNase-treated controls to rule out DNA-mediated protein aggregation .
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In imaging: Use Airyscan super-resolution microscopy to distinguish between nucleolar and cytoplasmic signals.
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Data interpretation: Normalize antibody signal intensity to histone H3 for nuclear proteins or actin for cytoplasmic fractions.
