ggact.1 Antibody

Definition and Target Protein

• GGACT Protein: Encoded by the GGACT gene, this enzyme catalyzes the conversion of gamma-glutamylamines to free amines and 5-oxoproline, playing a role in protein catabolism . It is orthologous across species, including humans, mice, rats, and zebrafish (e.g., ggact.1 in zebrafish) .

• Antibody Target: The antibody binds to the AA 1-50 region of human A2LD1 (GGACT) , with predicted cross-reactivity in dog, horse, and rabbit .

Applications

The GGACT antibody is validated for multiple techniques:

Cross-Reactivity and Species Specificity

• Confirmed Reactivity: Human, mouse, rat .

• Predicted Cross-Reactivity: Dog, horse, rabbit (based on sequence homology) .

• Zebrafish ggact.1: While no commercial antibodies explicitly target zebrafish ggact.1, cross-reactivity can be assessed via BLAST analysis of the immunogen sequence .

Research Findings

• Protein Function: GGACT degrades isodipeptide cross-links formed by transglutaminases, aiding in fibrinolysis .

• Subcellular Localization: Predicted to be cytosolic, with membrane-associated regions .

• Tissue Expression: Enriched in kidney and brain, with low specificity in other tissues .

Future Directions

• Zebrafish Studies: Development of antibodies targeting zebrafish ggact.1 could enhance research on fibrin degradation pathways in aquatic models .

• Therapeutic Potential: GGACT inhibitors may modulate proteolytic pathways in diseases involving cross-linked proteins .