GULLO1 Antibody

What is GULLO1 Antibody?

GULLO1 Antibody is a polyclonal antibody targeting the probable L-gulonolactone oxidase 1 (GULLO1) protein in Arabidopsis thaliana (Mouse-ear cress). This enzyme, encoded by the GULLO1 gene (At1g32300), belongs to the oxidoreductase family (EC 1.1.3.8) and is implicated in metabolic pathways involving L-gulonolactone, a precursor in ascorbic acid biosynthesis . The antibody is designed for specific detection and quantification of GULLO1 in experimental assays such as ELISA and Western Blot (WB) .

Research Applications

GULLO1 Antibody is primarily utilized in plant biology to investigate metabolic pathways and enzyme functions. Key applications include:

Key Research Findings

• Enzymatic Role: GULLO1 is a mitochondrial enzyme involved in redox reactions, with homology to L-gulonolactone oxidases in other species .

• Expression Patterns: Studies using this antibody have linked GULLO1 to stress-response pathways in Arabidopsis, though detailed mechanistic insights remain under investigation .

• Validation: The antibody demonstrates high sensitivity in WB, detecting GULLO1 at concentrations as low as 0.1 µg/mL .

Future Directions

Current research gaps include elucidating GULLO1’s role in ascorbate metabolism and its interaction with other redox enzymes. The antibody’s utility in CRISPR-edited plant models could further clarify its biological significance .