GULLO5 Antibody
Description
Database and Literature Review
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PLAbDab (Patent and Literature Antibody Database) :
This repository contains 150,000+ antibody sequences from patents and studies. No entries match "GULLO5" in nomenclature or functional annotations. -
YCharOS Antibody Characterization Database :
Covers 812 antibodies with knockout validation data. "GULLO5" is absent from their reports. -
CiteAb’s Top 100 Research Antibodies (2022) :
No antibodies named "GULLO5" appear in commercial or research antibody listings.
Potential Explanations for Absence
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Typographical Error: The name may be misspelled or conflated with known antibodies (e.g., "GULO" refers to gulonolactone oxidase, a human gene).
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Proprietary Name: If "GULLO5" is an internal code for an undisclosed therapeutic candidate, public data may be restricted.
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Emerging Research: The compound might be in early preclinical stages without published results.
Recommendations for Further Investigation
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Verify Nomenclature: Cross-check spelling and naming conventions with the HUGO Gene Nomenclature Committee (HGNC) or the WHO’s International Nonproprietary Names (INN) system.
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Explore Patent Databases: Search the USPTO or WIPO databases for unpublished patent applications.
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Contact Developers: If "GULLO5" is a proprietary asset, reach out to the originating institution or company for details.
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
Q&A
Based on the analysis of research literature and antibody development methodologies, here is a structured FAQ addressing key aspects of GLUT5 (SLC2A5) antibody research. The questions are categorized by complexity and supported by experimental evidence from peer-reviewed studies.
Advanced Research Questions
How to resolve cross-reactivity with structurally similar glucose transporters?
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Implement combinatorial array screening to assess antibody binding against GLUT family members (GLUT1-14). Use surface plasmon resonance to quantify dissociation constants (KD) for each transporter .
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For in vivo studies, combine antibody staining with transporter-specific inhibitors (e.g., phloretin for GLUT2) to isolate GLUT5-specific signals .
What experimental designs enable epitope characterization of GLUT5 antibodies?
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Use alanine scanning mutagenesis guided by the AB-Bind database to identify critical binding residues .
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For structural analysis:
How to analyze conflicting data between Western blot and IHC results?
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Apply a systematic troubleshooting framework:
Discrepancy Type Root Cause Analysis Resolution Strategy Positive WB/Negative IHC Epitope masking in fixed tissue Test multiple fixation protocols Variable band intensities Post-translational modifications Use deglycosylation enzymes (PNGase F) Species-specific reactivity Evolutionary divergence Validate across 3+ species
Methodological Considerations
What high-throughput approaches exist for GLUT5 antibody screening?
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Implement NGS-linked functional screening:
