Customize At2g18620 Antibody

Description

Definition and Basic Characteristics

The At2g18620 Antibody (Product Code: CSB-PA124356XA01DOA) is a polyclonal antibody raised against the protein encoded by the At2g18620 locus in Arabidopsis thaliana. This gene is annotated in the UniProt database under accession Q9ZU77, though its precise molecular function remains under investigation. The antibody is produced in standardized volumes (2 mL or 0.1 mL) and is validated for applications such as Western blotting, immunohistochemistry, and ELISA .

Research Applications

The At2g18620 Antibody is primarily utilized to:

Map Protein Expression: Identify spatial and temporal expression patterns of the At2g18620 protein in Arabidopsis tissues .
Study Subcellular Localization: Determine cellular compartments where the protein accumulates (e.g., nucleus, cytoplasm) via immunofluorescence .
Investigate Protein Interactions: Support pull-down assays to identify binding partners or post-translational modifications .

Comparative Data on Related Antibodies

The At2g18620 Antibody belongs to a broader catalog of Arabidopsis-targeting reagents. Below is a subset of related antibodies for context :

Product Name	Target Gene	UniProt ID	Applications
GONST2 Antibody	GONST2	Q84L09	WB, IHC
GOLS10 Antibody	GOLS10	F4KED2	ELISA, IF
At2g18620 Antibody	At2g18620	Q9ZU77	WB, IHC, ELISA

Challenges and Future Directions

Functional Annotation: The At2g18620 protein lacks detailed functional characterization, necessitating studies using knockout mutants alongside antibody-based assays .
Technical Optimization: Advances in antibody engineering, such as affinity maturation or epitope tagging, could improve detection limits .

Validation and Quality Control

The antibody’s specificity is typically confirmed using:

Knockout Controls: Comparison of signal in wild-type vs. At2g18620 mutant plants.
Peptide Blocking: Pre-incubation with the immunizing peptide to abolish signal .

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

At2g18620 antibody; F24H14.3Geranylgeranyl pyrophosphate synthase 7 antibody; chloroplastic antibody; GGPP synthase 7 antibody; GGPS7 antibody; EC 2.5.1.-; antibody; 2E,6E)-farnesyl diphosphate synthase 7 antibody; Dimethylallyltranstransferase 7 antibody; EC 2.5.1.1 antibody; Farnesyl diphosphate synthase 7 antibody; Farnesyltranstransferase 7 antibody; EC 2.5.1.29 antibody; Geranyltranstransferase 7 antibody; EC 2.5.1.10 antibody

Target Names

At2g18620

Uniprot No.

Q9ZU77

Target Background

Function

This antibody targets At2g18620, an enzyme that catalyzes the trans-addition of three molecules of isopentenyl pyrophosphate (IPP) onto dimethylallyl pyrophosphate (DMAPP) to form geranylgeranyl pyrophosphate.

Database Links

KEGG: ath:AT2G18620

STRING: 3702.AT2G18620.1

UniGene: At.52813

Protein Families

FPP/GGPP synthase family

Subcellular Location

Plastid, chloroplast.

Q&A

Here’s a structured collection of FAQs tailored for academic researchers working with the At2g18620 antibody, integrating methodological guidance and data-driven insights from peer-reviewed studies:

Advanced Research Questions

What structural features of At2g18620 influence antibody-antigen binding?
- Use deep-learning models (trained on antibody-antigen interfaces, as in ) to predict epitope regions.
- Perform alanine scanning mutagenesis on At2g18620 to identify critical binding residues.
How do I design a study to assess At2g18620’s role in carotenoid biosynthesis?
- Experimental workflow:
  1. Generate transgenic Arabidopsis lines overexpressing At2g18620.
  2. Quantify carotenoids via HPLC (methods in ).
  3. Correlate expression levels (via qPCR/Western blot) with metabolite profiles.
- Key controls: Use mutants defective in downstream enzymes (e.g., lycopene cyclase ).
What computational tools can enhance At2g18620 antibody validation?
- Implement graph convolutional networks (as in ) to predict antibody-antigen interaction strength.
- Use molecular dynamics simulations to model binding stability under varying pH/temperature conditions.

Table 1: Comparison of Antibody Validation Methods

Method	Sensitivity	Specificity	Throughput	Key Application
Immunoblotting	Moderate	High	Low	Initial specificity screening
Surface Plasmon Resonance (SPR)	High	Very High	Medium	Affinity quantification
ELISA	High	Moderate	High	Cross-reactivity profiling

Table 2: Common Cross-Reactive Species for At2g18620 Antibody

Species	Homology (%)	Likelihood of Cross-Reactivity
Brassica oleracea	89%	High
Arabidopsis lyrata	95%	Very High
Glycine max	72%	Low

Methodological Best Practices

For epitope mapping: Combine hydrogen-deuterium exchange mass spectrometry (HDX-MS) with SPR-based binding assays (as in ) to resolve conformational epitopes.
To address batch variability: Pre-absorb antibodies against lysates from knockout models to remove nonspecific binders.
In functional studies: Use dual-labeling immunofluorescence with organelle-specific markers to localize At2g18620 in planta.

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At2g18620 Antibody