GIF2 Antibody

GIF Antibodies: Definition and Context

GIF (glycosylation-inhibiting factor) is a 13-kDa cytokine involved in immunosuppression by inhibiting N-glycosylation of IgE-binding factors (IgE-BF), thereby suppressing IgE synthesis . GIF antibodies are designed to detect and study the function of this cytokine in immune regulation.

Immunosuppressive Mechanisms of GIF

GIF suppresses both IgE and IgG1 antibody responses by modulating T-cell priming and IgE-BF glycosylation . Mutated recombinant GIF derivatives (e.g., C57A/N106S) exhibit enhanced bioactivity compared to wild-type GIF:

Data adapted from .

Antibody Applications in GIF Detection

GIF antibodies enable precise detection of GIF expression in cellular and biochemical assays:

Clone 012 (Alexa Fluor® 405)

• Applications: Immunoprecipitation, Western blot .

• Detection:

  • Immunoprecipitation: Effective at 1:25–1:100 dilution.

  • Western Blot: Detects 13-kDa bands in human cell lysates.

Clone 007 (Unconjugated)

• Applications: Immunocytochemistry, flow cytometry .

• Validation:

  • Flow Cytometry: Detects GIF on HeLa cells with FITC-conjugated secondaries.

  • Immunofluorescence: Cytoplasmic localization in fixed HeLa cells.

GIF Antibody Engineering and Stability

While not directly related to GIF antibodies, studies on IgG2 subclass engineering highlight strategies applicable to antibody design:

Limitations and Future Directions

1. Nomenclature: The term "GIF2" is not standardized; it may refer to a specific isoform, mutant, or proprietary variant not yet published.

2. Therapeutic Potential: While GIF derivatives suppress IgE/IgG responses, clinical applications require optimization of bioavailability and safety .

3. Assay Optimization: Cross-reactivity and aggregation risks (observed in IgG2 antibodies ) necessitate careful conjugation and storage protocols .