At3g04190 Antibody
Description
Applications in Research
The antibody is validated for use in standard immunological techniques:
| Application | Details |
|---|---|
| ELISA | Detects At3g04190 in antigen-specific binding assays |
| Western Blot | Identifies the protein in lysates or membrane preparations |
Note: Confirm specificity in experimental conditions (e.g., tissue type, buffer compatibility) .
Research Findings and Functional Insights
The At3g04190 protein is a member of the germin-like protein (GLP) family, which plays critical roles in plant defense and stress responses .
Gene and Protein Characteristics
| Gene Name | Accession | Protein Function |
|---|---|---|
| At3g04190 | Q9M8X5 | Defense-related processes (e.g., pathogen resistance) |
Key Findings:
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Defense Mechanisms: GLPs, including At3g04190, are implicated in apoplast fluid defense, where they interact with pathogens or environmental stresses .
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Hormone Regulation: GLP expression is influenced by auxins (e.g., IAA), suggesting a role in hormone-mediated stress responses .
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Salt Tolerance: Transgenic plants overexpressing GLP homologs (e.g., GmGER9 in soybean) show enhanced salt resistance, indicating potential conserved functions .
Antibody Utility: The At3g04190 Antibody facilitates the detection of this protein in:
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Proteomic Studies: Identifying GLPs in apoplast fluids or cellular lysates .
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Subcellular Localization: Mapping protein distribution via immunofluorescence (not explicitly tested but inferred from similar antibodies) .
Challenges and Considerations
While the antibody is a valuable tool, its effective use requires careful validation:
Critical Insight: Antibody performance can vary across applications; orthogonal methods (e.g., immunocapture mass spectrometry) should complement immunological assays .
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
