APL2 Antibody

Research Applications

• Western Blot: Validated for detecting endogenous APPL2 in human and mouse samples .

• Immunohistochemistry: Used to localize APPL2 in tissue sections.

• Functional Studies: APPL2 is implicated in insulin signaling, glucose metabolism, and endosomal trafficking. Its role in modulating adiponectin signaling and AMP-activated protein kinase (AMPK) pathways has been highlighted in metabolic studies .

Validation and Quality Control

• Specificity: Confirmed via knockout/knockdown validation in relevant cell lines.

• Batch Consistency: Rigorous antigen-affinity purification ensures minimal lot-to-lot variability.

• Cross-Reactivity: Predicts reactivity across species with high sequence homology to human APPL2 .

Key Findings in APPL2 Research

• Metabolic Regulation: APPL2 interacts with adiponectin receptors to regulate glucose uptake and fatty acid oxidation.

• Subcellular Localization: Localizes to endosomal compartments, influencing signal transduction pathways like Akt and MAPK .

Comparative Note: APL-2 (Complement Inhibitor)

While APPL2 Antibody (14294-1-AP) is a research tool, the similarly named APL-2 is a distinct compound—a synthetic PEGylated cyclic peptide developed by Apellis Pharmaceuticals. APL-2 inhibits complement C3 and is in clinical trials for complement-mediated diseases (e.g., paroxysmal nocturnal hemoglobinuria, autoimmune hemolytic anemia) .

References

1. Proteintech. (2025). APPL2 Antibody (14294-1-AP). https://www.ptglab.com/products/APPL2-Antibody-14294-1-AP.htm

2. Apellis Pharmaceuticals. (2025). Phase 3 Study of APL-2. https://investors.apellis.com/news-releases

3. PMC. (2023). Anti-β2GPI/HLA-DR Antibodies in Thrombosis. https://pmc.ncbi.nlm.nih.gov/articles/PMC10557208/