GLIP2 Antibody

GLI-2 Antibody (AF3526)

Target: GLI-2, a transcription factor critical for Hedgehog signaling and regulation of genes involved in cell proliferation, differentiation, and survival.

Key Features

Research Findings

• ChIP Applications: Used to identify GLI-2-regulated genes in Jurkat T-cells treated with PMA and calcium ionomycin. DNA complexes immunoprecipitated with AF3526 were validated for GLI-2 binding sites .

• Citations:

  • SOX2 regulation in melanoma: GLI-2 antibodies identified its role in maintaining self-renewal and tumorigenicity of melanoma-initiating cells .

  • Cytokine cross-talk in tumors: GLI-2 mediates interactions between cytokines and the tumor microenvironment, influencing immune responses .

GLP-2R Antibody (MAB4285)

Target: GLP-2R, a G-protein-coupled receptor involved in intestinal homeostasis and energy absorption.

Key Features

Research Findings

• Flow Cytometry: Stains human GLP-2R transfectants but not irrelevant controls (e.g., non-transfected cells) .

• Functional Studies:

  • Neuroendocrine signaling: GLP-2R antibodies aid in studying GLP-2 feedback mechanisms in colon cancer, linking WNT signaling to retinoic acid pathways .

  • Gastrointestinal hormone responses: Used in engineering human iPSC-derived parasympathetic neurons to enhance hormone responsiveness .

Comparative Analysis of GLI-2 and GLP-2R Antibodies

Broader Context: Antibody Databases and Computational Tools

While "GLIP2" is not a recognized target, modern antibody research leverages databases like PLAbDab (Patent and Literature Antibody Database), which curates 150,000 paired antibody sequences from diverse sources (patents, literature, structures) . These resources enable:

• Sequence-based searches (e.g., KA-search for sequence identity) .

• Structural comparisons (e.g., RoseTTAfold for antibody-antigen docking) .

• Functional annotations, including antigen specificity and clinical applications .