GLB1 Antibody
Description
Senescence Marker
GLB1 is a validated marker for senescence-associated β-galactosidase (SA-β-gal) activity, distinguishing it from other lysosomal enzymes . In prostate cancer (PCa), elevated GLB1 expression correlates with:
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Lower tumor stage (p = 0.01)
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Localized vs. metastatic disease (p = 0.0003)
Detection in Formalin-Fixed Paraffin-Embedded (FFPE) Tissues
GLB1 antibodies enable quantitative imaging in FFPE samples using platforms like Vectra and AQUA. Key findings include:
| Tissue Type | GLB1 Expression | Senescence Correlation |
|---|---|---|
| High-grade PIN (HGPIN) | High (72% positive) | Strong (p < 0.01 vs. benign) |
| Primary PCa | Moderate (37% positive) | Inverse with metastasis (p = 0.003) |
| Benign Prostate | Low (13% positive) | None |
Data adapted from PLOS ONE studies .
Prostate Cancer Prognostics
In intermediate-grade PCa (Gleason 6–7), high GLB1 expression predicts:
| Outcome | Low GLB1 | High GLB1 | p-value |
|---|---|---|---|
| PSA-Free Survival (Days) | 629 | 2314 | 0.013 |
| Tumor Heterogeneity | High | Low | <0.001 |
Adapted from survival analyses in PLOS ONE .
Product Overview
GLB1 antibodies are available as polyclonal (rabbit) or monoclonal (mouse) reagents, with varying reactivities:
Key Features
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Sensitivity: Detects both active and inactive GLB1 isoforms.
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Specificity: Minimal cross-reactivity with non-lysosomal β-galactosidases.
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Validation: Tested in replicative senescence models and therapy-induced senescence (e.g., AZQ-treated PCa cells) .
Related Research: GLB1 Homologue in Autoimmunity
A study on Glb1 homologue antibodies (targeting a wheat protein homolog) found no association with islet autoimmunity in type 1 diabetes but noted:
| Factor | Association | p-value |
|---|---|---|
| Breastfeeding Duration | Inverse (cases only) | 0.001 |
| Gluten Intake | Direct (cases only) | 0.007 |
| Zonulin (gut permeability) | Direct (cases only) | 0.003 |
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- The GLB1 protein plays a significant role in fine-tuning fatty acid biosynthesis and partitioning in seeds. (PMID: 21070409)
- Research has shown that the function of the GLB1 protein has evolved between bacterial and plant systems. It controls the carbon metabolism pathway of fatty acid synthesis in plastids by regulating acetyl-CoA carboxylase activity. (PMID: 20018655)
- Studies provide evidence for a subtle involvement of the GLB1 protein in the regulation of certain steps in primary carbon and nitrogen metabolism. (PMID: 16133214)
- The major Arabidopsis PII-interacting protein has been identified as the chloroplast-localized enzyme N-acetyl glutamate kinase, which plays a key regulatory role in the pathway of arginine biosynthesis. (PMID: 16377628)
- In vitro assays using purified recombinant proteins have demonstrated the catalytic activation of Arabidopsis N-acetyl glutamate kinase (NAGK) by the GLB1 protein, providing the first evidence of its physiological role in higher plants. (PMID: 16545809)
- The crystal structure reveals a highly conserved segment at the N-terminus, found exclusively in plant GLB1 proteins, which interacts with the alpha2 helix and extends from the surface of the homotrimer opposite to the T-loop. (PMID: 17279613)
- Knockout mutants lacking the GLB1 gene exhibit an increased light-dependent nitrite uptake into chloroplasts. (PMID: 18325336)
