At2g13100 Antibody

What is the At2g13100 Antibody?

The At2g13100 antibody is a polyclonal antibody developed to recognize the protein encoded by the At2g13100 gene in Arabidopsis thaliana. This gene is annotated as part of the phosphate starvation-induced glycerol-3-phosphate permease (G3Pp) family, which plays a role in phosphate transport and metabolism under nutrient-limiting conditions .

Biological Role of the At2g13100 Gene

The At2g13100 gene encodes a glycerol-3-phosphate permease (G3Pp) homolog, which is induced during phosphate starvation. This protein family facilitates the uptake of glycerol-3-phosphate, a critical metabolite in lipid biosynthesis and phosphate scavenging under nutrient-deficient conditions .

Functional Insights:

• Phosphate Acquisition: G3Pp proteins enable plants to utilize organic phosphate sources, such as glycerol-3-phosphate, when inorganic phosphate is scarce.

• Stress Adaptation: Expression is upregulated during phosphate deprivation, highlighting its role in stress response .

Research Applications of the At2g13100 Antibody

While direct studies using this antibody are not detailed in publicly available literature, its utility can be inferred from the gene’s functional annotations:

Potential Use Cases:

• Localization Studies: Tracking subcellular distribution of the G3Pp protein under varying phosphate conditions.

• Expression Profiling: Quantifying protein levels during phosphate starvation or genetic manipulation (e.g., knockout mutants).

• Interaction Networks: Identifying binding partners via co-immunoprecipitation.

Challenges and Considerations

• Specificity Validation: Commercial antibodies (e.g., angiotensin receptor antibodies) often show nonspecific binding in Western blots . Independent validation of the At2g13100 antibody using At2g13100 knockout lines is critical.

• Functional Studies: No published data directly link this antibody to mechanistic studies, underscoring the need for further research.

Future Directions

• Characterization: Detailed biochemical assays to confirm target specificity.

• Phenotypic Analysis: Linking protein expression to phosphate uptake efficiency in transgenic plants.

• Comparative Studies: Exploring homologs in crops to improve phosphate-use efficiency in agriculture.