GLR2.2 Antibody

Immunohistochemistry and Immunofluorescence

• L21/32 (DSHB) shows strong reactivity in rat brain sections, particularly in neuronal populations expressing GluR2 .

• AGC-005 (Alomone) enables live-cell imaging of GluR2 on the surface of neurons, critical for studying receptor dynamics .

Western Blotting

• L21/32 detects a ~90 kDa band corresponding to GluR2 in rat brain lysates .

• AGC-005 confirms specificity by blocking peptide competition, eliminating non-specific signals .

Functional Studies

• 6C4 (Merck Millipore) has been used to study AMPA receptor composition in synaptic plasticity and cognitive flexibility .

• Knockout (KO) validation in mice confirms that L21/32 does not cross-react with GluA1, GluA3, or GluA4 .

4. Key Research Findings
Recent studies highlight the role of GluR2 antibodies in elucidating receptor function and pathology:

Challenges and Controversies

• Specificity concerns: Some antibodies show cross-reactivity with homologous receptors (e.g., GluA1, GluA3) .

• Diagnostic limitations: Despite initial reports, GluD2 antibodies are not reliable biomarkers for opsoclonus-myoclonus syndrome (OMS) .