GLR2.4 Antibody
Description
Structure and Function of the GLRAntibody
The GLR2.4 antibody is derived from rabbit hosts and purified via antigen-affinity chromatography . Its structure follows the classical Y-shaped IgG configuration, characterized by:
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Fab regions: Variable domains (V<sub>H</sub> and V<sub>L</sub>) responsible for antigen recognition .
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Fc region: Constant domains mediating interactions with immune effector systems .
While specific epitope mapping for GLR2.4 is not publicly disclosed, its polyclonal nature ensures broad reactivity to the target protein’s epitopes, enhancing detection sensitivity in assays like Western blot (WB) and enzyme-linked immunosorbent assay (ELISA) .
Applications in Research
The GLR2.4 antibody is primarily employed in:
Gene Family Context
GLR2.4 belongs to the AtGLR gene family in Arabidopsis, which encodes 20 glutamate receptor-like proteins divided into three clades . Phylogenetic analyses suggest GLR2.4 is part of clade II, which includes receptors implicated in amino acid transport and signaling .
Expression Patterns
RT-PCR studies reveal GLR2.4 is expressed in multiple tissues, with normalized expression levels varying by organ:
| Tissue | Normalized Expression (Relative to TUB5) | Key Findings |
|---|---|---|
| Roots | Moderate (0.6–0.8) | May regulate nutrient uptake. |
| Leaves | High (0.8–1.0) | Linked to photosynthesis or stress responses. |
| Flowers | Low (0.2–0.4) | Potential role in reproductive signaling. |
Potential Limitations and Future Directions
While the GLR2.4 antibody enables robust detection of the target protein, several challenges remain:
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Specificity: Polyclonal antibodies may cross-react with homologous GLR family members (e.g., GLR2.1, GLR2.3) .
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Quantification: ELISA requires rigorous calibration to avoid false positives.
Future studies could leverage CRISPR-Cas9 or RNAi to knock down GLR2.4 expression, validating the antibody’s specificity and functional relevance .
Product Specs
Target Background
KEGG: ath:AT4G31710
STRING: 3702.AT4G31710.1
Q&A
Based on analysis of peer-reviewed studies on antibody research methodologies and receptor targeting, here is a structured FAQ addressing key scientific considerations for GLR2.4 antibody research:
Advanced Research Questions
How to resolve contradictions between in vitro binding data and in vivo functional outcomes?
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Analysis Framework:
Case Study Insights
In anti-TLR2 antibody development, 68% of candidates showing <5 nM KD in SPR failed in murine inflammation models due to:
What computational strategies optimize antibody paratope-receptor interface?
How to validate antibody-mediated receptor internalization without fluorescent tags?
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Label-free Methodology:
