GLR2.6 Antibody

Introduction

The GLR2.6 antibody is a tool used in scientific research, particularly in the study of glutamate receptors . Glutamate receptors are vital for excitatory neurotransmission in animals . Antibodies like GLR2.6 can help scientists understand the structure, function, and interactions of these receptors .

Target and Specificity

GLR2.6 antibody targets a specific region of the GLR-2 glutamate receptor. GLR-2 is an AMPA receptor subunit in C. elegans . The antibody is designed to bind to this specific region, enabling researchers to identify and isolate GLR-2 for further study .

Applications in Research

GLR2.6 antibody is used in several techniques:

• Immunoprecipitation: GLR2.6 can be used to isolate GLR-2 protein from cell lysates. This allows researchers to study the proteins that interact with GLR-2 .

• Western Blotting: Following immunoprecipitation, western blotting can be used to confirm the presence of GLR-2 and identify interacting proteins .

• Immunocytochemistry: Antibodies can be used to visualize the location of GLR-2 within cells and tissues .

Interactions with MAGI-1

Research has shown that GLR-2 interacts with MAGI-1, a membrane-associated guanylate kinase (MAGUK) protein . Specifically, the GLR-2 cytoplasmic tail interacts with the MAGI-1L isoform . This interaction is dependent on the PDZ binding motif in the GLR-2 cytoplasmic domain .

Functional Significance

The interaction between GLR-2 and MAGI-1L modulates short-term habituation in response to long-term prior experience . This suggests that GLR-2 and MAGI-1L play a role in synaptic plasticity and learning .

Considerations for Use

When using GLR2.6 antibody, researchers should consider the following:

• Purity: It is important to ensure that the antibody is of high purity to minimize non-specific binding .

• Titer: The antibody should have a high enough titer to ensure sufficient binding to the target protein .

• Validation: The antibody should be validated for the specific application, such as western blotting or immunoprecipitation .