GLR3.2 Antibody
Description
Molecular Identity and Functional Role of GLR3.2
GLR3.2 belongs to clade III of the plant glutamate receptor-like (GLR) family, which shares structural homology with animal ionotropic glutamate receptors (iGluRs) but exhibits distinct ligand-binding properties . Key features include:
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Structure: Contains a bilobed ligand-binding domain (LBD) that adopts a clamshell conformation, activated by amino acids like glycine and asparagine .
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Localization: Primarily localized to the phloem of Arabidopsis roots, particularly near sieve plates .
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Function: Forms heteromeric channels with GLR3.4 to regulate calcium influx, influencing lateral root primordia development and positioning .
Applications of GLRAntibody in Research
The GLR3.2 antibody has been instrumental in:
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Protein Localization Studies: Immunohistochemistry and GFP-tagged constructs revealed phloem-specific expression in roots .
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Interaction Analysis: Co-immunoprecipitation and biophotonic assays demonstrated physical interaction between GLR3.2 and GLR3.4 .
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Mutant Phenotyping: Characterized glr3.2 knockout mutants, which exhibit hyperproduction of lateral root primordia due to disrupted calcium signaling .
Key Research Findings
The table below summarizes studies utilizing GLR3.2-targeted approaches:
Implications for Plant Biology
GLR3.2 research highlights its role in:
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Root Development: Restricting lateral root primordia overproduction via phloem-localized signaling .
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Stress Responses: Potential involvement in abiotic stress adaptation through Ca²⁺-mediated signaling .
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Evolutionary Conservation: Structural parallels with iGluRs underscore conserved gating mechanisms across kingdoms .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Two genes, AT2G17260 and AT4G35290, exhibit a RAV1-A binding site motif in their promoters. This, coupled with gene ontology annotation, suggests their involvement in stomatal movement through abscisic acid signaling. PMID: 27632363
- Studies indicate that in roots, GLR3.4 and GLR3.2 are primarily localized in the phloem, particularly in the vicinity of the sieve plates. GLR3.3, on the other hand, is expressed in most cells of the growing primary root but is not enriched in the phloem. PMID: 23590882
