GLR3.6 Antibody

Introduction to GLRAntibody

The GLR3.6 antibody is a specialized immunological tool designed to target and detect the Arabidopsis thaliana Glutamate Receptor-Like Channel 3.6 (GLR3.6), a member of the plant glutamate receptor-like (GLR) family. GLR3.6 plays critical roles in root development, wound signaling, and stress responses . This antibody enables researchers to study GLR3.6’s expression, localization, and interaction networks, providing insights into its molecular mechanisms.

Development and Validation of GLRAntibody

GLR3.6 antibodies are typically raised against specific epitopes within the protein’s extracellular or intracellular domains. Validation includes:

• Specificity: Western blotting confirms recognition of the ~110 kDa GLR3.6 protein in wild-type Arabidopsis, absent in glr3.6 mutants .

• Localization: Immunostaining in root tissues validates plasma membrane and vascular tissue localization, consistent with GLR3.6’s role in root meristem regulation .

• Functional assays: Antibody-blocking experiments demonstrate reduced root growth and impaired Ca²⁺ signaling, aligning with GLR3.6’s physiological roles .

Table 1: Research Applications of GLR3.6 Antibody

Mechanistic Insights from GLRStudies

• Root Development: GLR3.6 regulates root meristem size by modulating mitotic activity via the cell cycle inhibitor KRP4 .

• Phosphorylation: Calcium-dependent protein kinase CDPK16 phosphorylates GLR3.6 at Ser856, enhancing its interaction with 14-3-3ω to regulate ion channel activity .

• Wound Signaling: GLR3.6 in phloem companion cells mediates leaf-to-leaf electrical signals, requiring functional Ca²⁺ channel activity .

Case Study 1: Root Meristem Regulation

• Method: GLR3.6 antibody-based immunolocalization in glr3.6 mutants vs. wild type.

• Result: Mutants showed reduced meristem size and KRP4 upregulation, reversed by KRP4 knockdown .

Case Study 2: Phosphorylation-Dependent Interactions

• Method: Co-IP using GLR3.6 antibody identified 14-3-3ω as a binding partner.

• Result: Ser861/Ser862 phosphorylation is essential for this interaction, confirmed by BiFC assays .

Challenges and Limitations

• Cross-Reactivity: Potential cross-reactivity with clade 3 GLRs (e.g., GLR3.4, GLR3.7) due to sequence homology .

• Dynamic Expression: GLR3.6 levels vary under stress, requiring careful normalization in quantitative assays .

Future Directions

• Structural Studies: Cryo-EM of GLR3.6-antibody complexes to map epitopes .

• Translational Research: Engineering stress-tolerant crops via GLR3.6 modulation .